M
Maja Kiselinova
Researcher at Ghent University
Publications - 27
Citations - 634
Maja Kiselinova is an academic researcher from Ghent University. The author has contributed to research in topics: Digital polymerase chain reaction & Viral load. The author has an hindex of 14, co-authored 26 publications receiving 545 citations. Previous affiliations of Maja Kiselinova include Ghent University Hospital & Maastricht University.
Papers
More filters
Journal ArticleDOI
Comparison of droplet digital PCR and seminested real-time PCR for quantification of cell-associated HIV-1 RNA.
Maja Kiselinova,Alexander O. Pasternak,Ward De Spiegelaere,Dirk Vogelaers,Ben Berkhout,Linos Vandekerckhove +5 more
TL;DR: Quantitative assays for CA HIV RNA have the potential to improve monitoring of patients on ART and to be used in clinical studies aimed at HIV eradication, but should be cross-validated by multiple laboratories prior to wider use.
Journal ArticleDOI
ddpcRquant: threshold determination for single channel droplet digital PCR experiments
Wim Trypsteen,Matthijs Vynck,Jan De Neve,Pawel Bonczkowski,Maja Kiselinova,Eva Malatinkova,Karen Vervisch,Olivier Thas,Olivier Thas,Linos Vandekerckhove,Ward De Spiegelaere +10 more
TL;DR: This work shows that the normality assumption that the fluorescent signal of droplets is normally distributed does not likely hold true for most ddPCR runs, resulting in an erroneous threshold, and suggests a methodology that does not make any assumptions about the distribution of the fluorescence readouts.
Journal ArticleDOI
High-throughput Characterization of HIV-1 Reservoir Reactivation Using a Single-Cell-in-Droplet PCR Assay.
Robert W. Yucha,Kristen S. Hobbs,Emily Hanhauser,Louise E. Hogan,Wildaliz Nieves,Mehmet Ozgun Ozen,Fatih Inci,Vanessa A. York,Erica A. Gibson,Cassandra Thanh,Hadi Shafiee,Hadi Shafiee,Rami El Assal,Maja Kiselinova,Yvonne P. Robles,Helen Bae,Kaitlyn S. Leadabrand,ShuQi Wang,Steven G. Deeks,Daniel R. Kuritzkes,Daniel R. Kuritzkes,Utkan Demirci,Timothy J. Henrich +22 more
TL;DR: A microfluidic single-cell-in-droplet (scd)PCR assay is developed to directly measure the number of CD4+ T cells that produce unspliced (us)RNA and multiply spliced (ms)RNA following ex vivo latency reversal with either an histone deacetylase inhibitor (romidepsin) or T cell receptor (TCR) stimulation.
Journal ArticleDOI
Diagnostic utility of droplet digital PCR for HIV reservoir quantification.
TL;DR: In the context of a low level of detection and HIV reservoir diagnostics, ddPCR can offer a valid alternative to qPCR-based assays but before this platform can be clinically accredited, some remaining issues need to be resolved.
Journal ArticleDOI
During Stably Suppressive Antiretroviral Therapy Integrated HIV-1 DNA Load in Peripheral Blood is Associated with the Frequency of CD8 Cells Expressing HLA-DR/DP/DQ
Alessandra Ruggiero,Ward De Spiegelaere,Alessandro Cozzi-Lepri,Maja Kiselinova,Georgios Pollakis,Apostolos Beloukas,Linos Vandekerckhove,Matthew C. Strain,Douglas D. Richman,Andrew N. Phillips,Anna Maria Geretti,Paola Vitiello,Nicola Mackie,Jonathan Ainsworth,Anele Waters,Frank A. Post,Simon Edwards,Julie Fox +17 more
TL;DR: The observed positive association between integrated HIV-1 DNA load and frequency of CD8+DR/DP/DQ+ cells indicates that a close correlation between HIV persistence and immune activation continues during consistently suppressive therapy.