M
Makoto Handa
Researcher at Keio University
Publications - 153
Citations - 5660
Makoto Handa is an academic researcher from Keio University. The author has contributed to research in topics: Platelet & Platelet membrane glycoprotein. The author has an hindex of 43, co-authored 150 publications receiving 5403 citations. Previous affiliations of Makoto Handa include Niigata University & Oregon Health & Science University.
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Journal ArticleDOI
The role of von Willebrand factor and fibrinogen in platelet aggregation under varying shear stress.
Yasuo Ikeda,Makoto Handa,Koichi Kawano,Tetsuji Kamata,Mitsuru Murata,Y. Araki,Hironobu Anbo,Y. Kawai,K Watanabe,Ichiro Itagaki +9 more
TL;DR: Von Willebrand factor in the absence of exogenous agonists can mediate platelet aggregation in experimental conditions that may mimic the hemorheological situation of partially occluded arteries and may play a relevant role in thrombogenesis.
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The von Willebrand factor-binding domain of platelet membrane glycoprotein Ib. Characterization by monoclonal antibodies and partial amino acid sequence analysis of proteolytic fragments
TL;DR: The binding domain for von Willebrand factor resides near the NH2 terminus of the G PIb alpha-chain, whereas the carbohydrate-rich region is part of the innermost portion of GPIb and does not appear to be involved in the von Wilrebrand factor binding function.
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Turbulence activates platelet biogenesis to enable clinical scale ex vivo production
Ito Yukitaka,Sou Nakamura,Naoshi Sugimoto,Tomohiro Shigemori,Kato Yoshikazu,Mikiko Ohno,Shinya Sakuma,Keitaro Ito,Hiroki Kumon,Hidenori Hirose,Okamoto Haruki,Masayuki Nogawa,Mio Iwasaki,Shunsuke Kihara,Kosuke Fujio,Takuya Matsumoto,Natsumi Higashi,Kazuya Hashimoto,Akira Sawaguchi,Ken ichi Harimoto,Masato Nakagawa,Takuya Yamamoto,Makoto Handa,Naohide Watanabe,Eiichiro Nishi,Fumihito Arai,Satoshi Nishimura,Koji Eto,Koji Eto +28 more
TL;DR: This study provides a concept in which a coordinated physico-chemical mechanism promotes platelet biogenesis and an innovative strategy for ex vivo platelet manufacturing is provided.
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Thrombopoietin induces tyrosine phosphorylation of Stat3 and Stat5 in human blood platelets
Yoshitaka Miyakawa,Atsushi Oda,Brian J. Druker,Hiroshi Miyazaki,Makoto Handa,Hideya Ohashi,Yasuo Ikeda +6 more
TL;DR: The data indicate that Stat3 and Stat5 may be involved in signal transduction after ligand binding to c-Mpl and that this event may have a role in megakaryopoiesis/thrombopoiedis or possibly a mature platelet function such as aggregation.
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Transmembrane calcium influx associated with von Willebrand factor binding to GP Ib in the initiation of shear-induced platelet aggregation
Yasuo Ikeda,Makoto Handa,Tetsuji Kamata,Koichi Kawano,Yohko Kawai,Kiyoaki Watanabe,Kawakami Keiko,Kiyotaka Sakai,Mayumi Fukuyama,Ichiro Itagaki,Akira Yoshioka,Zaverio M. Ruggeri +11 more
TL;DR: The results suggest that the binding of multimeric vWF to GP Ib initiates platelets aggregation induced by high shear stress by mediating a transmembrane flux of Ca2+ ions, perhaps through a receptor-dependent calcium channel.