M
Malene Bech Vester-Christensen
Researcher at University of Copenhagen
Publications - 34
Citations - 2832
Malene Bech Vester-Christensen is an academic researcher from University of Copenhagen. The author has contributed to research in topics: Limit dextrinase & Glycosylation. The author has an hindex of 16, co-authored 33 publications receiving 2410 citations. Previous affiliations of Malene Bech Vester-Christensen include Technical University of Denmark & Novo Nordisk.
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Journal ArticleDOI
Precision mapping of the human O-GalNAc glycoproteome through SimpleCell technology
Catharina Steentoft,Sergey Y. Vakhrushev,Hiren J. Joshi,Hiren J. Joshi,Yun Kong,Malene Bech Vester-Christensen,Katrine T. Schjoldager,Kirstine Lavrsen,Sally Dabelsteen,Nis Borbye Pedersen,Lara Marcos-Silva,Lara Marcos-Silva,Ramneek Gupta,Eric P. Bennett,Ulla Mandel,Søren Brunak,Søren Brunak,Hans H. Wandall,Steven B. Levery,Henrik Clausen +19 more
TL;DR: A genetic engineering approach using human cell lines to simplify O‐glycosylation (SimpleCells) that enables proteome‐wide discovery of O-glycan sites using ‘bottom‐up’ ETD‐based mass spectrometric analysis and an improved NetOGlyc4.0 model for prediction of O‐ Glycoproteins.
Journal ArticleDOI
Mining the O-glycoproteome using zinc-finger nuclease-glycoengineered SimpleCell lines
Catharina Steentoft,Sergey Y. Vakhrushev,Malene Bech Vester-Christensen,Katrine T. Schjoldager,Yun Kong,Eric P. Bennett,Ulla Mandel,Hans H. Wandall,Steven B. Levery,Henrik Clausen +9 more
TL;DR: Zinc-finger nuclease gene targeting is applied to truncate the O-glycan elongation pathway in human cells, generating stable 'SimpleCell' lines with homogenous O- glycosylation and should facilitate analyses of important functions of protein gly cosylation.
Journal ArticleDOI
Engineered CHO cells for production of diverse, homogeneous glycoproteins.
Zhang Yang,Shengjun Wang,Adnan Halim,Morten Alder Schulz,Morten Frödin,Shamim Herbert Rahman,Malene Bech Vester-Christensen,Malene Bech Vester-Christensen,Carsten Behrens,Claus Kristensen,Sergey Y. Vakhrushev,Eric P. Bennett,Hans H. Wandall,Henrik Clausen +13 more
TL;DR: An engineering approach will aid the production of glycoproteins with improved properties and therapeutic potential by constructing a design matrix that facilitates the generation of desired glycosylation, such as human-like α2,6-linked sialic acid capping.
Journal ArticleDOI
Fast and sensitive detection of indels induced by precise gene targeting
Zhang Yang,Catharina Steentoft,Camilla Hauge,Lars Hansen,Allan Lind Thomsen,Francesco Niola,Malene Bech Vester-Christensen,Morten Frödin,Henrik Clausen,Hans H. Wandall,Eric P. Bennett +10 more
TL;DR: A method for fast, sensitive and simple indel detection that accurately defines indel sizes down to ±1 bp is presented, based on tri-primer amplicon labelling and DNA capillary electrophoresis detection and is amenable for high throughput analysis.
Journal ArticleDOI
Mining the O-mannose glycoproteome reveals cadherins as major O-mannosylated glycoproteins
Malene Bech Vester-Christensen,Adnan Halim,Hiren J. Joshi,Catharina Steentoft,Eric P. Bennett,Steven B. Levery,Sergey Y. Vakhrushev,Henrik Clausen +7 more
TL;DR: A unique strategy using nuclease-mediated gene editing of a human cell line to reduce the structural heterogeneity of O-Man glycans and to probe the O- man glycoproteome is presented, and it is found that the important cadherin and plexin families of cell membrane receptors are O-mannosylated.