S
Sergey Y. Vakhrushev
Researcher at University of Copenhagen
Publications - 97
Citations - 5038
Sergey Y. Vakhrushev is an academic researcher from University of Copenhagen. The author has contributed to research in topics: Glycosylation & Glycan. The author has an hindex of 32, co-authored 82 publications receiving 3862 citations. Previous affiliations of Sergey Y. Vakhrushev include University of Münster & University of Zagreb.
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Journal ArticleDOI
Precision mapping of the human O-GalNAc glycoproteome through SimpleCell technology
Catharina Steentoft,Sergey Y. Vakhrushev,Hiren J. Joshi,Hiren J. Joshi,Yun Kong,Malene Bech Vester-Christensen,Katrine T. Schjoldager,Kirstine Lavrsen,Sally Dabelsteen,Nis Borbye Pedersen,Lara Marcos-Silva,Lara Marcos-Silva,Ramneek Gupta,Eric P. Bennett,Ulla Mandel,Søren Brunak,Søren Brunak,Hans H. Wandall,Steven B. Levery,Henrik Clausen +19 more
TL;DR: A genetic engineering approach using human cell lines to simplify O‐glycosylation (SimpleCells) that enables proteome‐wide discovery of O-glycan sites using ‘bottom‐up’ ETD‐based mass spectrometric analysis and an improved NetOGlyc4.0 model for prediction of O‐ Glycoproteins.
Journal ArticleDOI
Mining the O-glycoproteome using zinc-finger nuclease-glycoengineered SimpleCell lines
Catharina Steentoft,Sergey Y. Vakhrushev,Malene Bech Vester-Christensen,Katrine T. Schjoldager,Yun Kong,Eric P. Bennett,Ulla Mandel,Hans H. Wandall,Steven B. Levery,Henrik Clausen +9 more
TL;DR: Zinc-finger nuclease gene targeting is applied to truncate the O-glycan elongation pathway in human cells, generating stable 'SimpleCell' lines with homogenous O- glycosylation and should facilitate analyses of important functions of protein gly cosylation.
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Immature truncated O-glycophenotype of cancer directly induces oncogenic features
Prakash Radhakrishnan,Sally Dabelsteen,Frey Brus Madsen,Chiara Francavilla,Katharina L. Kopp,Catharina Steentoft,Sergey Y. Vakhrushev,Jesper V. Olsen,Lars Hansen,Eric P. Bennett,Anders Woetmann,Guangliang Yin,Longyun Chen,Haiyan Song,Mads Bak,Ryan A. Hlady,Staci L. Peters,Rene Opavsky,Christenze Thode,Klaus Qvortrup,Katrine T. Schjoldager,Henrik Clausen,Michael A. Hollingsworth,Hans H. Wandall +23 more
TL;DR: It is demonstrated, with the use of well-defined cell systems generated by precise gene editing, that the aberrant O-glycophenotype by itself induces oncogenic features with enhanced growth and invasion, and further provides support for immunotherapeutic strategies that target aberrant o-glycans.
Journal ArticleDOI
Engineered CHO cells for production of diverse, homogeneous glycoproteins.
Zhang Yang,Shengjun Wang,Adnan Halim,Morten Alder Schulz,Morten Frödin,Shamim Herbert Rahman,Malene Bech Vester-Christensen,Malene Bech Vester-Christensen,Carsten Behrens,Claus Kristensen,Sergey Y. Vakhrushev,Eric P. Bennett,Hans H. Wandall,Henrik Clausen +13 more
TL;DR: An engineering approach will aid the production of glycoproteins with improved properties and therapeutic potential by constructing a design matrix that facilitates the generation of desired glycosylation, such as human-like α2,6-linked sialic acid capping.
Journal ArticleDOI
An Atlas of Human Glycosylation Pathways Enables Display of the Human Glycome by Gene Engineered Cells
Yoshiki Narimatsu,Hiren J. Joshi,Rebecca Nason,Julie Van Coillie,Richard Karlsson,Lingbo Sun,Zilu Ye,Yen-Hsi Chen,Katrine T. Schjoldager,Catharina Steentoft,Sanae Furukawa,Barbara A. Bensing,Paul M. Sullam,Andrew J. Thompson,James C. Paulson,Christian Büll,Christian Büll,Gosse J. Adema,Ulla Mandel,Lars Hansen,Eric P. Bennett,Ajit Varki,Sergey Y. Vakhrushev,Zhang Yang,Henrik Clausen +24 more
TL;DR: Broad utility of the cell-based glycan array is demonstrated, and the higher order binding of microbial adhesins to clustered patches of O-glycans organized by their presentation on proteins is uncovered.