M
Markus Axmann
Researcher at Max Planck Society
Publications - 11
Citations - 884
Markus Axmann is an academic researcher from Max Planck Society. The author has contributed to research in topics: T-cell receptor & Major histocompatibility complex. The author has an hindex of 9, co-authored 10 publications receiving 786 citations. Previous affiliations of Markus Axmann include Johannes Kepler University of Linz & Vienna University of Technology.
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Journal ArticleDOI
TCR–peptide–MHC interactions in situ show accelerated kinetics and increased affinity
Johannes B. Huppa,Markus Axmann,Manuel A. Mörtelmaier,Manuel A. Mörtelmaier,Manuel A. Mörtelmaier,Björn F. Lillemeier,Björn F. Lillemeier,Evan W. Newell,Mario Brameshuber,Lawrence O. Klein,Gerhard J. Schütz,Mark M. Davis,Mark M. Davis +12 more
TL;DR: It is shown that synaptic TCR–pMHC binding dynamics differ significantly from TCR’s binding in solution, and TCR affinity for pMHC was significantly elevated as the result of a large (about 100-fold) increase in the association rate, a likely consequence of complementary molecular orientation and clustering.
Journal ArticleDOI
Bax monomers form dimer units in the membrane that further self-assemble into multiple oligomeric species.
Yamunadevi Subburaj,Katia Cosentino,Markus Axmann,Esteban Pedrueza-Villalmanzo,Eduard Hermann,Stephanie Bleicken,Joachim P. Spatz,Ana J. García-Sáez +7 more
TL;DR: By analysing the stoichiometry of Bax oligomers at the single-molecule level, it is found that Bax binds to the membrane in a monomersic state and then self-assembles in <1 min, and active Bax does not exist in a unique oligomeric state, but as several different species based on dimer units.
Journal ArticleDOI
T Cell Activation is Determined by the Number of Presented Antigens
Janosch Deeg,Janosch Deeg,Markus Axmann,Markus Axmann,Jovana Matic,Jovana Matic,Anastasia Liapis,David Depoil,Jehan Afrose,Silvia Curado,Michael L. Dustin,Michael L. Dustin,Joachim P. Spatz,Joachim P. Spatz +13 more
TL;DR: N nanopatterned antigen arrays that mimic the antigen presenting cell surface during T cell activation are introduced and it is demonstrated that these substrates induce T cell responses in a pMHC dose-dependent manner and that the number of presented p MHCs dominates over local pM HC density.
Journal ArticleDOI
Versatile Analysis of Single-Molecule Tracking Data by Comprehensive Testing against Monte Carlo Simulations
TL;DR: An approach for the analysis of single-molecule trajectories which is based on a comprehensive comparison of an experimental data set with multiple Monte Carlo simulations of the diffusion process allows quantitative data analysis, particularly whenever analytical treatment of a model is infeasible.
Journal ArticleDOI
Determination of Interaction Kinetics between the T Cell Receptor and Peptide-Loaded MHC Class II via Single-Molecule Diffusion Measurements
Markus Axmann,Johannes B. Huppa,Mark M. Davis,Mark M. Davis,Gerhard J. Schütz,Gerhard J. Schütz +5 more
TL;DR: A single-molecule approach is presented to determine pMHC-TCR interaction kinetics in situ based on diffusion analysis of dye-labeled pM HC and finds synaptic off-rates >10-fold accelerated when compared to the dissociation of purified proteins measured in vitro.