Mario Brameshuber

TL;DR: It is shown that synaptic TCR–pMHC binding dynamics differ significantly from TCR’s binding in solution, and TCR affinity for pMHC was significantly elevated as the result of a large (about 100-fold) increase in the association rate, a likely consequence of complementary molecular orientation and clustering.

TL;DR: In this paper, a single-molecule imaging technique that uses quantum-dot-labeled peptide-major histocompatibility complex (pMHC) ligands was developed to study CD4 + T-cell functional sensitivity.

TL;DR: The results indicate that phase partitioning is not a fundamental element of protein organization in the plasma membrane and find that immobilized mGFP-GPIs behave as inert obstacles to the diffusion of other membrane constituents without influencing their membrane environment over distances beyond their physical size.

TL;DR: The classic heat shock (stress) response (HSR) was originally attributed to protein denaturation, but it is observed that a deregulated HSR is found in a large number of important diseases where more detailed knowledge of the molecular mechanisms involved may offer timely opportunities for clinical interventions and new, innovative drug treatments.

TL;DR: This work presents here a method that allows for the first time the direct imaging of nanoscopic long-lived platforms with raft-like properties diffusing in the live cell plasma membrane, and finds cholesterol-dependent homo-association in the plasma membrane of living CHO and Jurkat T cells in the resting state, thereby demonstrating the existence of small, mobile, long- lived platforms containing these probes.