Matthias Eibauer

TL;DR: A procedure, based upon focused ion beam (FIB) milling for the preparation of thin lamellae from vitrified cells grown on electron microscopy (EM) grids, which are apparently free of distortions or other artefacts and open up large windows into the cell’s interior allowing tomographic studies to be performed on any chosen part of the cell.

TL;DR: In this article, a detailed view of the organization of the lamin meshwork within the lamina was obtained using cryo-electron tomography, which showed that A-and B-type lamins assemble into tetrameric filaments of 3.5 nm thickness.

TL;DR: Here, correlative cryo-fluorescence microscopy is used to navigate large cellular volumes and to localize specific cellular targets and it is shown that the selected targets in frozen-hydrated specimens can be accessed directly by focused ion beam milling.

TL;DR: This work reconstructs the Xenopus laevis oocyte NPC from native nuclear envelopes up to 20 Å resolution by cryo-electron tomography in conjunction with subtomogram averaging and proposes a model for the architecture of the molecular gate at its central channel.

TL;DR: An algorithm is able to correctly align a single-tilt tomographic series without the help of fiducial markers thanks to the detection of thousands of small image patches that can be tracked over a short number of images in the series.