Ohad Medalia

TL;DR: Electron tomography of vitrified cells is a noninvasive three-dimensional imaging technique that opens up new vistas for exploring the supramolecular organization of the cytoplasm, focusing on the actin cytoskeleton of Dictyostelium cells.

TL;DR: The central plug/transporter of nuclear pore complexes was variable in volume and could occupy different positions along the nucleocytoplasmic axis, which supports the notion that it essentially represents cargo in transit.

TL;DR: This Meeting Review describes the proceedings and conclusions from the inaugural meeting of the Electron Microscopy Validation Validation Task Force organized by the Unified Data Resource for 3DEM and aims to increase the impact of 3DEM in biology and medicine.

TL;DR: Cryo-electron tomography is used to study the structure of nuclear pore complexes in their functional environment, that is, in intact nuclei of Dictyostelium discoideum and a new image-processing strategy compensating for deviations of the asymmetric units from a perfect eight-fold symmetry enabled the structure to be refined and to identify new features.

TL;DR: In this article, a detailed view of the organization of the lamin meshwork within the lamina was obtained using cryo-electron tomography, which showed that A-and B-type lamins assemble into tetrameric filaments of 3.5 nm thickness.