M
Melissa P. Lokugamage
Researcher at Georgia Institute of Technology
Publications - 26
Citations - 796
Melissa P. Lokugamage is an academic researcher from Georgia Institute of Technology. The author has contributed to research in topics: Medicine & Biology. The author has an hindex of 9, co-authored 16 publications receiving 322 citations. Previous affiliations of Melissa P. Lokugamage include Georgia Tech Research Institute & University of Missouri.
Papers
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Journal ArticleDOI
High-throughput in vivo screen of functional mRNA delivery identifies nanoparticles for endothelial cell gene editing.
Cory D. Sago,Melissa P. Lokugamage,Kalina Paunovska,Daryll Vanover,Christopher M. Monaco,Nirav N. Shah,Marielena Gamboa Castro,Shannon E. Anderson,Tobi G. Rudoltz,Gwyneth N. Lando,Pooja M. Tiwari,Jonathan L. Kirschman,Nick J. Willett,Young C. Jang,Philip J. Santangelo,Anton V. Bryksin,James E. Dahlman +16 more
TL;DR: A high-throughput method to measure how >100 nanoparticles delivered mRNA that was translated into functional protein in vivo and identify 7C2 and 7C3, two LNPs that efficiently deliver siRNA, single-guide RNA (sgRNA), and mRNA to endothelial cells.
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Nanoparticles Containing Oxidized Cholesterol Deliver mRNA to the Liver Microenvironment at Clinically Relevant Doses.
Kalina Paunovska,Alejandro J. Da Silva Sanchez,Cory D. Sago,Zubao Gan,Melissa P. Lokugamage,Fatima Z. Islam,Sujay Kalathoor,Brandon R. Krupczak,James E. Dahlman +8 more
TL;DR: It is found that an LNP formulated with oxidized cholesterol and no targeting ligand delivers Cre mRNA, which edits DNA in hepatic endothelial cells and Kupffer cells at 0.05 mg kg−1, suggesting that LNPs formulated with modified cholesterols can deliver gene‐editing mRNA to the liver microenvironment at clinically relevant doses.
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Constrained Nanoparticles Deliver siRNA and sgRNA to T Cells In Vivo without Targeting Ligands
TL;DR: The delivery of siRNA to 9 cell types in vivo by 168 nanoparticles using a novel siGFP‐based barcoding system and bioinformatics is quantified and it is found that nanomaterials containing conformationally constrained lipids form stable LNPs, herein named constrained lipid nanoparticles (cLNPs).
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Analyzing 2000 in Vivo Drug Delivery Data Points Reveals Cholesterol Structure Impacts Nanoparticle Delivery
Kalina Paunovska,Carmen J. Gil,Melissa P. Lokugamage,Cory D. Sago,Manaka Sato,Gwyn N. Lando,Marielena Gamboa Castro,Anton V. Bryksin,James E. Dahlman +8 more
TL;DR: Lipid nanoparticles formulated with esterified cholesterol delivered nucleic acids more efficiently than LNPs formulated with regular or oxidized cholesterol when compared across all tested cell types in the mouse, and an LNP containing cholesteryl oleate was identified that efficiently delivered siRNA and sgRNA to liver endothelial cells in vivo.
Journal ArticleDOI
Nanoparticles That Deliver RNA to Bone Marrow Identified by in Vivo Directed Evolution.
Cory D. Sago,Melissa P. Lokugamage,Fatima Z. Islam,Brandon R. Krupczak,Manaka Sato,James E. Dahlman +5 more
TL;DR: BM1 is the first nanoparticle to efficiently deliver siRNA and sgRNA to BMECs in vivo, demonstrating that this functional in vivo screen can identify nanoparticles with novel tropism in vivo and helping scientists understand how simple chemical changes control nanoparticle targeting.