M
Michael C. Alley
Researcher at Mayo Clinic
Publications - 33
Citations - 5165
Michael C. Alley is an academic researcher from Mayo Clinic. The author has contributed to research in topics: In vivo & In vitro. The author has an hindex of 23, co-authored 33 publications receiving 5021 citations. Previous affiliations of Michael C. Alley include Erasmus University Rotterdam.
Papers
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Journal Article
Feasibility of Drug Screening with Panels of Human Tumor Cell Lines Using a Microculture Tetrazolium Assay
Michael C. Alley,Dominic A. Scudiero,Anne Monks,Miriam L. Hursey,Maciej Czerwinski,Donald L. Fine,B. J. Abbott,Joseph G. Mayo,Robert H. Shoemaker,Michael R. Boyd +9 more
TL;DR: Since the microculture tetrazolium assay provides sensitive and reproducible indices of growth as well as drug sensitivity in individual cell lines over the course of multiple passages and several months' cultivation, it appears suitable for initial-stage in vitro drug screening.
Journal ArticleDOI
In vivo cultivation of tumor cells in hollow fibers.
Melinda G. Hollingshead,Michael C. Alley,Richard F. Camalier,B. J. Abbott,Joseph G. Mayo,Louis Malspeis,Michael R. Grever +6 more
TL;DR: Results of experimentation to date demonstrate that a hollow fiber encapsulation/implantation methodology provides quantitative indices of drug efficacy with minimum expenditures of time and materials.
Book ChapterDOI
Human Tumor Xenograft Models in NCI Drug Development
Jacqueline Plowman,Donald J. Dykes,Melinda G. Hollingshead,Linda Simpson-Herren,Michael C. Alley +4 more
TL;DR: The present chapter provides a brief history of the in vivo screens used by the NCI, a description of the human tumor xenograft systems that are employed in preclinical drug development, and a discussion of how these Xenograft models are employed for both initial efficacy testing as well as detailed drug evaluations.
Journal ArticleDOI
In vivo antitumor efficacy of 17-DMAG (17-dimethylaminoethylamino-17-demethoxygeldanamycin hydrochloride), a water-soluble geldanamycin derivative.
Melinda G. Hollingshead,Michael C. Alley,Angelika M. Burger,Suzanne Borgel,Christine M. Pacula-Cox,Heinz-Herbert Fiebig,Edward A. Sausville +6 more
TL;DR: The in vivo activity of 17-DMAG supports the further development of this water-soluble and potentially orally administrable geldanamycin congener.
Journal ArticleDOI
Cucurbitacin E-induced disruption of the actin and vimentin cytoskeleton in prostate carcinoma cells
TL;DR: The hypothesis that cucurbitacin treatment leads to an inappropriate increase in the filamentous or polymerized actin fraction in prostate carcinoma cells is supported and it is concluded that cucURbitacins are potent disruptors of cytoskeletal integrity.