M
Michael Liem
Researcher at La Trobe University
Publications - 15
Citations - 1875
Michael Liem is an academic researcher from La Trobe University. The author has contributed to research in topics: Microvesicles & Protein subunit. The author has an hindex of 14, co-authored 15 publications receiving 1415 citations.
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Journal ArticleDOI
Comparative proteomics evaluation of plasma exosome isolation techniques and assessment of the stability of exosomes in normal human blood plasma
Hina Kalra,Christopher G. Adda,Michael Liem,Ching-Seng Ang,Adam Mechler,Richard J. Simpson,Mark D. Hulett,Suresh Mathivanan +7 more
TL;DR: The OptiPrepTM density gradient method was superior in isolating pure exosomal populations, devoid of highly abundant plasma proteins, and in the context of cellular uptake, the isolated exosomes were able to fuse with target cells revealing that they were indeed biologically active.
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A novel mechanism of generating extracellular vesicles during apoptosis via a beads-on-a-string membrane structure
Georgia K. Atkin-Smith,Rochelle Tixeira,Stephanie Paone,Suresh Mathivanan,Christine Collins,Michael Liem,Katharine J. Goodall,Kodi S. Ravichandran,Mark D. Hulett,Ivan K. H. Poon +9 more
TL;DR: In an unbiased drug screen, the ability of sertraline (an antidepressant) to block the formation of ‘beads-on-a-string' protrusions and apoptotic bodies is identified and also compounds that can modulate this process are identified.
Journal ArticleDOI
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Shivakumar Keerthikumar,Lahiru Gangoda,Michael Liem,Pamali Fonseka,Ishara Atukorala,Cemil Ozcitti,Adam Mechler,Christopher G. Adda,Ching-Seng Ang,Suresh Mathivanan +9 more
TL;DR: It is ascertained that cancer cells facilitate oncogenesis by the secretion of mutant and oncoproteins into the tumor microenvironment via exosomes and ectosomes.
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Extracellular vesicles including exosomes are mediators of signal transduction: are they protective or pathogenic?
TL;DR: The involvement of EVs as mediators of signal transduction in neurodegenerative diseases and cancer is discussed and the role of EVs in mediating Wnt and PI3K signaling pathways is also discussed.
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Bovine milk-derived exosomes from colostrum are enriched with proteins implicated in immune response and growth.
Monisha Samuel,David Chisanga,Michael Liem,Shivakumar Keerthikumar,Sushma Anand,Ching-Seng Ang,Christopher G. Adda,Ellen Versteegen,Markandeya Jois,Suresh Mathivanan +9 more
TL;DR: The importance of exosomes in colostrum is highlighted and opens up new avenues to exploit these vesicles in the regulation of the immune response and growth.