Michelle M. Spiering

TL;DR: Recombinant holoNOS displayed similar spectral characteristics and activity as the enzyme isolated from murine macrophages and the initial inorganic product of the NADPH/O2 reaction is proposed to be the nitroxyl anion (NO-) based on the formation of a ferrous nitrosyl complex using the heme domain of soluble guanylate cyclase as a trap.

TL;DR: Together, the results suggest that important regulations occur within the replisome to achieve rapid and processive replication and that this weak helicase cannot efficiently unwind the T4 genome alone.

TL;DR: This work shows that enzymes in a cascade can assemble via chemotaxis, and finds that the chemotactic assembly of enzymes occurs even under cytosolic crowding conditions.

TL;DR: The role of DNA polymerase as a micropump opens up avenues for designing miniature fluid pumps using enzymes as engines.

TL;DR: The unwinding and priming activities of the bacteriophage T4 primosome, which consists of a hexameric helicase translocating 5′ to 3′ and an oligomeric primase synthesizing primers, is investigated on DNA hairpins manipulated by a magnetic trap to find that it continuously unwinds the DNA duplex while allowing for primer synthesis through a primosomes disassembly mechanism or a novel DNA looping mechanism.