T
Timothée Lionnet
Researcher at New York University
Publications - 67
Citations - 6072
Timothée Lionnet is an academic researcher from New York University. The author has contributed to research in topics: Chromatin & Transcription (biology). The author has an hindex of 31, co-authored 62 publications receiving 4879 citations. Previous affiliations of Timothée Lionnet include Yeshiva University & Howard Hughes Medical Institute.
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Journal ArticleDOI
A general method to improve fluorophores for live-cell and single-molecule microscopy
Jonathan B. Grimm,Brian P. English,Jiji Chen,Joel P Slaughter,Zhengjian Zhang,Andrey Revyakin,Ronak Patel,John J. Macklin,Davide Normanno,Robert H. Singer,Timothée Lionnet,Luke D. Lavis +11 more
TL;DR: Inspired by molecular modeling, the N,N-dimethylamino substituents in tetramethylrhodamine are replaced with four-membered azetidine rings, which doubles the quantum efficiency and improves the photon yield of the dye in applications ranging from in vitro single-molecule measurements to super-resolution imaging.
Journal ArticleDOI
Single-Molecule Dynamics of Enhanceosome Assembly in Embryonic Stem Cells
Jiji Chen,Zhengjian Zhang,Li M. Li,Bi-Chang Chen,Andrey Revyakin,Bassam Hajj,Wesley R. Legant,Maxime Dahan,Maxime Dahan,Timothée Lionnet,Eric Betzig,Robert Tjian,Zhe Liu +12 more
TL;DR: In vivo and in vitro single-molecule imaging, transcription factor (TF) mutagenesis, and ChIP-exo mapping are combined to determine how TFs dynamically search for and assemble on their cognate DNA target sites and find that enhanceosome assembly is hierarchically ordered.
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A transgenic mouse for in vivo detection of endogenous labeled mRNA
Timothée Lionnet,Kevin Czaplinski,Xavier Darzacq,Yaron Shav-Tal,Amber L. Wells,Jeffrey A. Chao,Hye Yoon Park,Valeria de Turris,Melissa Lopez-Jones,Robert H. Singer +9 more
TL;DR: This work generated a knock-in mouse line with an MS2 binding site (MBS) cassette targeted to the 3′ untranslated region of the essential ββ-actin gene, enabling live-cell imaging of single endogenous labeled mRNA molecules transcribed in primary mammalian cells and tissue.
Journal ArticleDOI
Bright photoactivatable fluorophores for single-molecule imaging
Jonathan B. Grimm,Brian P. English,Heejun Choi,Anand K. Muthusamy,Brian Mehl,Peng Dong,Timothy A. Brown,Jennifer Lippincott-Schwartz,Zhe Liu,Timothée Lionnet,Luke D. Lavis +10 more
TL;DR: This work refine and extend the utility of small, cell-permeable fluorophores by synthesizing photoactivatable derivatives that are compatible with live-cell labeling strategies, enabling improved single-particle tracking and facile localization microscopy experiments.
Journal ArticleDOI
Real-time quantification of single RNA translation dynamics in living cells
Tatsuya Morisaki,Kenneth Lyon,Keith F. DeLuca,Jennifer G. DeLuca,Brian P. English,Zhengjian Zhang,Luke D. Lavis,Jonathan B. Grimm,Sarada Viswanathan,Loren L. Looger,Timothée Lionnet,Timothy J. Stasevich +11 more
TL;DR: NCT, a technique that uses multi-epitope tags and antibody-based fluorescent probes to quantify protein synthesis dynamics at the single-mRNA level, reveals the stochastic nature of single mRNA translation.