M
Miguel A. Moreno-Mateos
Researcher at Yale University
Publications - 28
Citations - 2221
Miguel A. Moreno-Mateos is an academic researcher from Yale University. The author has contributed to research in topics: Zebrafish & Genome editing. The author has an hindex of 15, co-authored 24 publications receiving 1553 citations. Previous affiliations of Miguel A. Moreno-Mateos include University of Seville & Spanish National Research Council.
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Journal ArticleDOI
CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo
Miguel A. Moreno-Mateos,Charles E. Vejnar,Jean-Denis Beaudoin,Juan P. Fernandez,Emily K Mis,Mustafa K. Khokha,Antonio J. Giraldez +6 more
TL;DR: It is shown that targeting Cas9 to the germ line using a Cas9-nanos 3′ UTR led to the generation of maternal-zygotic mutants, as well as increased viability and decreased somatic mutations.
Journal ArticleDOI
Codon identity regulates mRNA stability and translation efficiency during the maternal‐to‐zygotic transition
Ariel A. Bazzini,Ariel A. Bazzini,Florencia del Viso,Miguel A. Moreno-Mateos,Timothy G. Johnstone,Charles E. Vejnar,Yidan Qin,Jun Yao,Mustafa K. Khokha,Antonio J. Giraldez +9 more
TL;DR: It is shown that the codon triplet contains translation‐dependent regulatory information that influences transcript decay, and shapes maternal mRNA clearance during the maternal‐to‐zygotic transition in zebrafish, Xenopus, mouse, and Drosophila, and gene expression during homeostasis across human tissues.
Journal ArticleDOI
CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing
Miguel A. Moreno-Mateos,Juan P. Fernandez,Romain Rouet,Romain Rouet,Charles E. Vejnar,Maura Lane,Emily K Mis,Mustafa K. Khokha,Jennifer A. Doudna,Antonio J. Giraldez +9 more
TL;DR: It is shown that temperature modulates Cpf1 activity by controlling its ability to access genomic DNA, and temperature is identified as a modulator of activity and used to efficiently edit ectothermic vertebrate species.
Journal ArticleDOI
CRISPR-Cas13d Induces Efficient mRNA Knockdown in Animal Embryos.
Gopal Kushawah,Luis Hernandez-Huertas,Joaquin Abugattas-Nuñez del Prado,Joaquin Abugattas-Nuñez del Prado,Juan R. Martinez-Morales,Michelle Lynn DeVore,Huzaifa Hassan,Ismael Moreno-Sanchez,Laura Tomas-Gallardo,Alejandro Díaz-Moscoso,Dahiana E. Monges,Javier R. Guelfo,William C. Theune,Emry O. Brannan,Wei Wang,Timothy J. Corbin,Andrea Moran,Alejandro Sánchez Alvarado,Edward Málaga-Trillo,Carter M. Takacs,Ariel A. Bazzini,Ariel A. Bazzini,Miguel A. Moreno-Mateos +22 more
TL;DR: It is shown that CRISPR-RfxCas13d (CasRx) is an effective and precise system to deplete specific mRNA transcripts in zebrafish embryos and can be used in medaka, killifish, and mouse embryos.
Journal ArticleDOI
Brd4 and P300 Confer Transcriptional Competency during Zygotic Genome Activation.
Shun Hang Chan,Yin Tang,Liyun Miao,Hiba Darwich-Codore,Charles E. Vejnar,Jean-Denis Beaudoin,Damir Musaev,Juan P. Fernandez,Maria D.J. Benitez,Ariel A. Bazzini,Ariel A. Bazzini,Miguel A. Moreno-Mateos,Antonio J. Giraldez +12 more
TL;DR: It is concluded that P300 and Brd4 are sufficient to trigger genome-wide transcriptional competency by regulating histone acetylation on the first zygotic genes in zebrafish, which is critical for initiatingZygotic development and developmental reprogramming.