Mike Sleutel

TL;DR: Experimental evidence shows that intermediate metastable states can be liquid-like clusters that are stable with respect to the parent liquid and metastable compared with the emerging crystalline phase and that clusters existing in protein solutions can significantly increase the nucleation rate and therefore play a relevant role in theucleation process.

TL;DR: It is shown that polymorph selection takes place at the earliest stages of structure formation and is based on specific building blocks for each space group, and ways of controlling macromolecular phase transitions are suggested, aiding the development of protein-based drug-delivery systems and macromolescular crystallography.

TL;DR: The data show that despite having all the characteristics typically associated with rich phase behaviour, glucose isomerase 2D crystals are formed classically, illustrating the resurfacing importance of the classical nucleation theory by re-validating some of the key assumptions that have been recently questioned.

TL;DR: Observations indicate that the protein-rich clusters are locations for crystal nucleation and that newly nucleated crystals within protein- rich clusters were detected directly.

TL;DR: The relative merits and domains of application of three observation techniques (atomic force microscopy, Michelson interferometry, and laser confocal microscopy with differential interference contrast microscopy) for the investigation of crystal growth kinetics are discussed in the context of protein crystallization.