Mikihiro Shibata

TL;DR: High-speed atomic force microscopy is used to visualize dynamic changes in stimulated proteins and confirms that high-resolution visualization is a powerful approach for studying elaborate biomolecular processes under realistic conditions.

TL;DR: High-speed atomic force micropcopy (HS-AFM) movies indicate that, whereas apo-Cas9 adopts unexpected flexible conformations, Cas9–RNA forms a stable bilobed structure and interrogates target sites on the DNA by three-dimensional diffusion.

TL;DR: The characterization of an oppositely directed H+ pump with a similar architecture to outward pumps is reported, demonstrating an elaborate molecular design to control the direction of H+ transfers in proteins.

TL;DR: Long-tip high-speed atomic force microscopy is capable of imaging morphogenesis of filopodia, membrane ruffles, pit formation, and endocytosis in COS-7, HeLa cells and hippocampal neurons.

TL;DR: A wide-area scanner with a maximum XY scan range of ~46 × 46 μm is developed by magnifying the displacements of stack piezoelectric actuators using a leverage mechanism and the nonlinearity of the X- and Y-piezoelectic actuators' displacements that arises from their hysteresis is eliminated by polynomial-approximation-based open-loop control.