Milton J. Zmijewski

TL;DR: The NAD(P)H-dependent enzymatic activity within living Zygosaccharomyces rouxii was utilized to accomplish this reaction and the reaction was scaled-up to a volume of 300 l by utilizing a commercially available agitated filter as a reactor.

TL;DR: Penicillin G amidase from E. coli has been shown to selectively acylate, in an efficient manner, the (2R,3S) isomer of a cis, racemic azetidinone intermediate used in a synthesis of loracarbef, a carbacephalosporin antibiotic.

TL;DR: Novel glycopeptides derived from teicoplanin were prepared and evaluated for activity against antibiotic-resistant gram-positive pathogens and some had potent activity against both staphylococci and glycopePTide-resistant enterococci.

TL;DR: Gel filtration studies demonstrated that all the enzyme activity eluted from the column at a molecular weight of 44 kDa, the first report of an enzyme activity that appears to be associated with the biosynthesis of glycopeptide antibiotics.

TL;DR: A novel ketoreductase isolated from Zygosaccharomyces rouxii catalyzes the asymmetric reduction of selected ketone substrates of commercial importance and substrate specificity, lack of a catalytic metal, and extent of protein sequence identity to known reductases suggests that the enzyme falls into the carbonyl reductase enzyme class.