Nathalie Eissing

TL;DR: It is demonstrated that DC subpopulations in organs of the lymphohematopoietic system (spleen, thymus, and blood) are strongly defined by ontogeny rather than by signals from the microenvironment, strongly arguing that DCs react toward modulatory signals from tissue microenvironments.

TL;DR: It is demonstrated that Ag targeting to the CD11c+CD8− DC subpopulation in the presence of stimulating anti-CD40 Ab and TLR3 ligand polyinosinic-polycytidylic acid induces protective responses against rapidly growing tumor cells in naive animals under preventive and therapeutic treatment regimens in vivo.

TL;DR: This work provides a protocol for the visualization of at least six differently fluorochrome-labeled antibodies at the same time using a conventional confocal laser scanning microscope with four laser lines in both murine and human tissue samples, and demonstrates that compensation correction algorithms are not necessary to reduce spillover of fluorochromes into other channels.

TL;DR: This work has found differential antigen presentation capacities of murine DC subpopulations using an in vivo antigen targeting system and the functional role of human tissue DCs is largely unknown.

TL;DR: In this article, a protocol for combining fluorochromes with the same excitation but different emission properties that allows for the analysis of six different antigens in confocal immunofluorescence microscopy with a conventional 4-laser line microscope is described.