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Neil G. Rumachik
Researcher at Thermo Fisher Scientific
Publications - 7
Citations - 215
Neil G. Rumachik is an academic researcher from Thermo Fisher Scientific. The author has contributed to research in topics: Sf9 & Monosaccharide. The author has an hindex of 4, co-authored 6 publications receiving 107 citations. Previous affiliations of Neil G. Rumachik include Stanford University.
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Journal ArticleDOI
An RNA-centric dissection of host complexes controlling flavivirus infection.
Yaw Shin Ooi,Karim Majzoub,Karim Majzoub,Ryan A. Flynn,Miguel A. Mata,Jonathan Diep,Jason Kenichi Li,Nicholas van Buuren,Neil G. Rumachik,Alex G. Johnson,Andreas S. Puschnik,Caleb D. Marceau,Luwanika Mlera,Jeffrey M. Grabowski,Karla Kirkegaard,Marshall E. Bloom,Peter Sarnow,Carolyn R. Bertozzi,Carolyn R. Bertozzi,Jan E. Carette +19 more
TL;DR: A survey of the cellular RNA-binding proteins that interact with dengue virus and Zika virus genomic RNA identifies ribosome-binding protein 1 and vigilin as bona fide RBPs able to promote viral RNA translation, replication and stability.
Journal ArticleDOI
Methods Matter: Standard Production Platforms for Recombinant AAV Produce Chemically and Functionally Distinct Vectors
Neil G. Rumachik,Stacy A. Malaker,Nicole Poweleit,Lucy H. Maynard,Christopher M. Adams,Ryan D. Leib,Giana Cirolia,Dennis R. Thomas,Susan Stamnes,Kathleen Holt,Patrick L. Sinn,Andrew P. May,Nicole K. Paulk +12 more
TL;DR: Differences in vector performance may have clinical implications for rAAV receptor binding, trafficking, expression kinetics, expression durability, vector immunogenicity, as well as cost considerations.
Journal ArticleDOI
Chemical Modulation of Protein O-GlcNAcylation via OGT Inhibition Promotes Human Neural Cell Differentiation.
Lissette M. Andres,Ian W. Blong,Angela C. Evans,Neil G. Rumachik,Teppei Yamaguchi,Nam D. Pham,Pamela Thompson,Jennifer J. Kohler,Carolyn R. Bertozzi,Carolyn R. Bertozzi +9 more
TL;DR: It is suggested that chemical inhibition of OGT and perturbation of protein O-GlcNAcylation accelerate the differentiation of hESCs along the neuronal lineage, thus providing further insight into the dynamic molecular mechanisms involved in neuronal development.
Posted ContentDOI
Methods Matter -- Standard Production Platforms For Recombinant AAV Can Produce Chemically And Functionally Distinct Vectors
Neil G. Rumachik,Neil G. Rumachik,Stacy A. Malaker,Nicole Poweleit,Lucy H. Maynard,Christopher M. Adams,Christopher M. Adams,Ryan D. Leib,Giana Cirolia,Dennis R. Thomas,Susan Stamnes,Kathleen Holt,Kathleen Holt,Patrick L. Sinn,Andrew P. May,Nicole K. Paulk +15 more
TL;DR: It is demonstrated that host cell species is critical for determining vector potency and human-produced rAAVs are more potent than baculovirus-Sf9 vectors in various cell types in vitro (P < 0.05-0.0001), in various mouse tissues in vivo, and in human liver in vivo.
Journal ArticleDOI
VectorMOD: Method for Bottom-Up Proteomic Characterization of rAAV Capsid Post-Translational Modifications and Vector Impurities.
TL;DR: In this paper, the authors present a simple step-by-step guide for academic rAAV laboratories and Chemistry, Manufacturing and Control (CMC) groups in industry to perform an in-house or outsourced bottom-up mass spectrometry workflow to characterize capsid PTMs and process impurities.