Nicolas Bery

TL;DR: This work describes the first fully synthetic phage display library of humanized llama single domain antibody (NaLi-H1: Nanobody Library Humanized 1), based on a humanized synthetic singledomain antibody (hs2dAb) scaffold optimized for intracellular stability, which provides high affinity binders without animal immunization.

TL;DR: Small molecules are identified that inhibit RAS-effector interactions and readily penetrate cells and demonstrate a general concept for developing small compounds to replace intracellular antibody fragments, enabling rational drug development to target validated PPIs.

TL;DR: Fusing the common, anchoring part from the two new compounds with the inhibitory substituents of the Abd series is created, and a set of compounds that inhibit RAS-effector interactions with increased potency are created.

TL;DR: DARPin K27 is a potent, non-covalent inhibitor of nucleotide exchange, showing consistent effects across different isoforms of Ras, including wild-type and oncogenic mutant forms.

TL;DR: Two designed ankyrin repeat proteins (DARPins) are characterised that specifically inhibit the KRAS isoform by binding to an allosteric site encompassing the region around KRAS-specific residue histidine 95 at the helix α3/loop 7/helix α4 interface.