N
Nils Krietenstein
Researcher at University of Massachusetts Medical School
Publications - 23
Citations - 1055
Nils Krietenstein is an academic researcher from University of Massachusetts Medical School. The author has contributed to research in topics: Chromatin & Nucleosome. The author has an hindex of 10, co-authored 18 publications receiving 678 citations. Previous affiliations of Nils Krietenstein include Ludwig Maximilian University of Munich.
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Journal ArticleDOI
Ultrastructural Details of Mammalian Chromosome Architecture
Nils Krietenstein,Sameer Abraham,Sergey V. Venev,Nezar Abdennur,Johan H. Gibcus,Tsung-Han S. Hsieh,Krishna Mohan Parsi,Liyan Yang,René Maehr,Leonid A. Mirny,Job Dekker,Oliver J. Rando +11 more
TL;DR: A recently developed Hi-C variant, Micro-C, is extended to map chromosome architecture at nucleosome resolution in human ESCs and fibroblasts, providing a valuable resource for studies of chromosome organization.
Journal ArticleDOI
Genomic Nucleosome Organization Reconstituted with Pure Proteins
Nils Krietenstein,Megha Wal,Shinya Watanabe,Bongsoo Park,Craig L. Peterson,B. Franklin Pugh,Philipp Korber +6 more
TL;DR: This work reconstitutes four stages of nucleosome architecture using purified components: yeast genomic DNA, histones, sequence-specific Abf1/Reb1, and remodelers RSC, ISW2, INO80, and ISW1a, and establishes core mechanisms by which promoter chromatin architecture arises through a blend of redundancy and specialization.
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CHD1 remodelers regulate nucleosome spacing in vitro and align nucleosomal arrays over gene coding regions in S. pombe
Julia Pointner,Jenna Persson,Punit Prasad,Ulrika Norman-Axelsson,Annelie Strålfors,Olga Khorosjutina,Nils Krietenstein,J. Peter Svensson,Karl Ekwall,Philipp Korber +9 more
TL;DR: The role of remodelers in global nucleosome positioning in S. pombe is studied and a striking evolutionary shift in remodeler usage between budding and fission yeast is found.
Journal ArticleDOI
Systematic evaluation of chromosome conformation capture assays.
Betul Akgol Oksuz,Liyan Yang,Sameer Abraham,Sergey V. Venev,Nils Krietenstein,Krishna Mohan Parsi,Hakan Ozadam,Hakan Ozadam,Marlies E. Oomen,Ankita Nand,Hui Mao,Ryan M.J. Genga,René Maehr,Oliver J. Rando,Leonid A. Mirny,Leonid A. Mirny,Johan H. Gibcus,Job Dekker,Job Dekker +18 more
TL;DR: In this paper, an improved Hi-C protocol (Hi-C 3.0) was proposed to detect both loops and compartments relatively effectively. But the performance of this protocol was limited by the cross-linking chemistry and chromatin fragmentation strategies.
Journal ArticleDOI
Absolute nucleosome occupancy map for the Saccharomyces cerevisiae genome.
Elisa Oberbeckmann,Michael Roland Wolff,Nils Krietenstein,Nils Krietenstein,Mark Heron,Jessica L Ellins,Andrea Schmid,Stefan Krebs,Helmut Blum,Ulrich Gerland,Philipp Korber +10 more
TL;DR: This work established two orthogonal and thereby crossvalidating approaches to measure absolute nucleosome occupancy across the Saccharomyces cerevisiae genome via restriction enzymes and DNA methyltransferases and provides a quantitative method and reference frame in absolute terms for future chromatin studies.