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Nina Costantino
Researcher at Weizmann Institute of Science
Publications - 40
Citations - 4668
Nina Costantino is an academic researcher from Weizmann Institute of Science. The author has contributed to research in topics: Recombineering & Homologous recombination. The author has an hindex of 21, co-authored 37 publications receiving 4270 citations.
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Journal ArticleDOI
Simple and highly efficient BAC recombineering using galK selection
TL;DR: Three new recombineering strains are described that allow bacterial artificial chromosomes (BACs) to be modified using galK positive/negative selection, and it is shown how galK selection can be used to rapidly introduce point mutations, deletions and loxP sites into BAC DNA and thus facilitate functional studies of SNP and/or disease-causing point mutations.
Reference EntryDOI
E. coli genome manipulation by P1 transduction.
TL;DR: This unit describes the procedure used to move portions of the E. coli genome from one genetic variant to another by the P1 bacteriophage.
Journal ArticleDOI
A set of recombineering plasmids for gram-negative bacteria
TL;DR: A set of plasmids that can be used to express recombineering functions in some gram-negative bacteria, thereby facilitating in vivo genetic manipulations are constructed, and it is anticipated that that they will provide efficient recombination in other related gram- negative bacteria.
Journal ArticleDOI
Recombineering: Genetic Engineering in Bacteria Using Homologous Recombination
Lynn C. Thomason,Donald L. Court,Mikail Bubunenko,Nina Costantino,Helen R. Wilson,Simanti Datta,Amos B. Oppenheim +6 more
TL;DR: Support protocols are presented that describe several two‐step selection/counter‐selection methods of making genetic alterations without leaving any unwanted changes in the targeted DNA, and a method for retrieving onto a plasmid a genetic marker from the Escherichia coli chromosome or a co‐electroporated DNA fragment.
Journal ArticleDOI
Enhanced levels of λ Red-mediated recombinants in mismatch repair mutants
Nina Costantino,Donald L. Court +1 more
TL;DR: The results show that Beta is the only bacteriophage function required for this level of recombination and suggest that Beta directs the ssDNA to the replication fork as it passes the target sequence.