Reference EntryDOI
E. coli genome manipulation by P1 transduction.
TLDR
This unit describes the procedure used to move portions of the E. coli genome from one genetic variant to another by the P1 bacteriophage.Abstract:
This unit describes the procedure used to move portions of the E. coli genome from one genetic variant to another. Fragments of approximately 100 kb can be transferred by the P1 bacteriophage. The phage is first grown on a strain containing the elements to be moved, and the resulting phage lysate is used to infect a second recipient strain. The lysate will contain bacterial DNA as well as phage DNA, and genetic recombination, catalyzed by enzymes of the recipient strain, will incorporate the bacterial fragments into the recipient chromosome.read more
Citations
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Journal ArticleDOI
Conversion of Escherichia coli to Generate All Biomass Carbon from CO2.
Shmuel Gleizer,Roee Ben-Nissan,Yinon M. Bar-On,Niv Antonovsky,Elad Noor,Yehudit Zohar,Ghil Jona,Eyal Krieger,Melina Shamshoum,Arren Bar-Even,Ron Milo +10 more
TL;DR: This work constructed and evolved Escherichia coli to produce all its biomass carbon from CO2 and achieved autotrophic growth following several months of continuous laboratory evolution in a chemostat under intensifying organic carbon limitation.
Journal ArticleDOI
Escherichia coli "Marionette" strains with 12 highly optimized small-molecule sensors.
TL;DR: A directed evolution approach was applied to optimize a set of 12 small-molecule-responsive biosensors, which led to the engineering of “Marionette” strains of Escherichia coli incorporating these sensors for biotechnological applications.
Journal ArticleDOI
A process for microbial hydrocarbon synthesis: Overproduction of fatty acids in Escherichia coli and catalytic conversion to alkanes.
TL;DR: A metabolically engineered strain of Escherichia coli is reported that overproduces medium‐chain length fatty acids via three basic modifications: elimination of β‐oxidation, overexpression of the four subunits of acetyl‐CoA carboxylase, and expression of a plant acyl–acyl carrier protein (ACP) thioesterase from Umbellularia californica (BTE).
Journal ArticleDOI
Fitness and stability of obligate cross-feeding interactions that emerge upon gene loss in bacteria
Samay Pande,Holger Merker,Katrin Bohl,Michael Reichelt,Stefan Schuster,Luis F. de Figueiredo,Christoph Kaleta,Christian Kost +7 more
TL;DR: In this paper, the authors investigated the effect of cross-feeding interactions in the presence of non-cooperating types by deleting two metabolic genes from the genome of Escherichia coli.
Journal ArticleDOI
Rho and NusG suppress pervasive antisense transcription in Escherichia coli.
Jason M. Peters,Rachel A. Mooney,Jeffrey A. Grass,Erik Jessen,Frances Tran,Robert Landick,Robert Landick +6 more
TL;DR: Global colocalization of the histone-like nucleoid-structuring protein (H-NS) with Rho-dependent terminators and genetic interactions between hns and rho suggest that H-NS aids Rho in suppression of antisense transcription.
References
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Journal ArticleDOI
Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection.
Tomoya Baba,Takeshi Ara,Miki Hasegawa,Yuki Takai,Yoshiko Okumura,Miki Baba,Kirill A. Datsenko,Masaru Tomita,Barry L. Wanner,Hirotada Mori,Hirotada Mori +10 more
TL;DR: These mutants—the ‘Keio collection’—provide a new resource not only for systematic analyses of unknown gene functions and gene regulatory networks but also for genome‐wide testing of mutational effects in a common strain background, E. coli K‐12 BW25113.
Journal ArticleDOI
Transduction of linked genetic characters of the host by bacteriophage P1
TL;DR: Transduction of characters between bacteria of the coli and dysentery groups indicates genetic homologies between these groups.
Book
A short course in bacterial genetics : a laboratory manual and handbook for Escherichia coli and related bacteria
TL;DR: Experiments in Molecular Genetics as mentioned in this paper is a spiral bound manual for those doing genetic or recombinant DNA work with E. coli or similar organisms with step-by-step protocols and clear diagrams that demonstrate major concep
Journal ArticleDOI
A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli.
Mitchell Singer,Tania A. Baker,G Schnitzler,S M Deischel,M Goel,W Dove,K J Jaacks,Alan D. Grossman,James W. Erickson,Carol A. Gross +9 more
TL;DR: A collection of 182 isogenic strains containing genetically linked antibiotic resistance elements located at approximately 1-min intervals around the Escherichia coli chromosome, designed to be used in a rapid two-step mapping system in E. coli.