O
Olga Pontes
Researcher at Washington University in St. Louis
Publications - 46
Citations - 7008
Olga Pontes is an academic researcher from Washington University in St. Louis. The author has contributed to research in topics: DNA methylation & RNA-Directed DNA Methylation. The author has an hindex of 30, co-authored 46 publications receiving 6485 citations. Previous affiliations of Olga Pontes include University of New Mexico & Instituto Superior de Agronomia.
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Journal ArticleDOI
Gateway-compatible vectors for plant functional genomics and proteomics.
Keith Earley,Jeremy R. Haag,Olga Pontes,Kristen E. Opper,Tom Juehne,Keming Song,Craig S. Pikaard +6 more
TL;DR: The utility of pEarleyGate destination vectors for the expression of epitope-tagged proteins that can be affinity captured or localized by immunofluorescence microscopy is demonstrated.
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Plant nuclear RNA polymerase IV mediates siRNA and DNA methylation-dependent heterochromatin formation.
Yasuyuki Onodera,Yasuyuki Onodera,Jeremy R. Haag,Thomas S. Ream,Pedro Costa Nunes,Olga Pontes,Craig S. Pikaard +6 more
TL;DR: In this article, the authors show that Pol IV helps produce siRNAs that target de novo cytosine methylation events required for facultative heter-chromatin formation and higher-order heter-romatin associations.
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The Arabidopsis Chromatin-Modifying Nuclear siRNA Pathway Involves a Nucleolar RNA Processing Center
Olga Pontes,Carey Fei Li,Pedro Costa Nunes,Pedro Costa Nunes,Jeremy R. Haag,Thomas S. Ream,Alexa Vitins,Steven E. Jacobsen,Craig S. Pikaard +8 more
TL;DR: It is hypothesized that maintenance of the heterochromatic state involves locus-specific Pol IVa transcription followed by siRNA production and assembly of AGO4- and NRPD1b-containing silencing complexes within nucleolar processing centers.
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An ARGONAUTE4-containing nuclear processing center colocalized with Cajal bodies in Arabidopsis thaliana.
Carey Fei Li,Olga Pontes,Mahmoud El-Shami,Ian R. Henderson,Yana V. Bernatavichute,Simon W. L. Chan,Thierry Lagrange,Craig S. Pikaard,Steven E. Jacobsen +8 more
TL;DR: The results suggest a function for Cajal bodies as a center for the assembly of an AGO4/NRPD1b/siRNA complex, facilitating its function in RNA-directed gene silencing at target loci.
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A Concerted DNA Methylation/Histone Methylation Switch Regulates rRNA Gene Dosage Control and Nucleolar Dominance
Richard J. Lawrence,Keith Earley,Olga Pontes,Manuela Silva,Z. Jeffrey Chen,N. Neves,Wanda Viegas,Craig S. Pikaard +7 more
TL;DR: The data support a model in which cytosine methylation and histone deacetylation are each upstream of one another in a self-reinforcing repression cycle.