Oren Parnas

TL;DR: Perturb-seq accurately identifies individual gene targets, gene signatures, and cell states affected by individual perturbations and their genetic interactions, and posit new functions for regulators of differentiation, the anti-viral response, and mitochondrial function during immune activation.

TL;DR: In this article, the authors introduced genome-wide pooled CRISPR-Cas9 libraries into dendritic cells (DCs) to identify genes that control the induction of tumor necrosis factor (Tnf) by bacterial lipopolysaccharide (LPS), a key process in the host response to pathogens, mediated by the Tlr4 pathway.

TL;DR: It is shown that yeast Elg1 interacts physically and genetically with PCNA, in a manner that depends on PCNA modification, and exhibits preferential affinity for SUMOylated PCNA.

TL;DR: It is concluded that PAHSA treatment regulates innate and adaptive immune responses to prevent mucosal damage and protect against colitis, and may be a novel treatment for colitis and related inflammation-driven diseases.

TL;DR: Evidence is presented that Elg1, Ctf4, and Ctf18 may coordinate the relative movement of the replication fork with respect to the cohesin ring, and that CTF18 and Elg 1 affect cohesion via a joint pathway.