Oumeng Zhang

TL;DR: A Tri-spot point spread function (PSF) is designed and implemented that simultaneously measures the three-dimensional orientation and the rotational mobility of dipole-like emitters across a large field of view and detects rotational dynamics of single molecules within a polymer thin film that are not observable by conventional SMLM.

TL;DR: A performance metric is reported, termed variance upper bound (VUB), that yields a global maximum CRB for all possible molecular orientations, thereby enabling the measurement performance of any PSF to be computed efficiently (~1000× faster than calculating average CRB).

TL;DR: SMOLM, single-molecule orientation localization microscopy, is utilized to directly measure the orientation spectra (3D orientation plus "wobble") of lipophilic probes transiently bound to lipid membranes, revealing that Nile red's (NR) orientation specta are extremely sensitive to membrane chemical composition.

TL;DR: In this paper, a mathematical framework was proposed to compute the fundamental limit of accuracy in measuring the rotational mobility of dipole-like emitters, which can be applied to both in-plane and three-dimensional methods.

TL;DR: In this paper, the authors compare the performance of multiple state-of-the-art and commonly used methods for orientation localization microscopy against the fundamental limits of measurement precision, revealing optimal imaging methods for various experiment conditions and sample geometries.