P
Pablo Huertas
Researcher at Spanish National Research Council
Publications - 60
Citations - 3784
Pablo Huertas is an academic researcher from Spanish National Research Council. The author has contributed to research in topics: Homologous recombination & DNA repair. The author has an hindex of 26, co-authored 54 publications receiving 3291 citations. Previous affiliations of Pablo Huertas include University of Cambridge & Pablo de Olavide University.
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Journal ArticleDOI
Cotranscriptionally Formed DNA:RNA Hybrids Mediate Transcription Elongation Impairment and Transcription-Associated Recombination
Pablo Huertas,Andrés Aguilera +1 more
TL;DR: It is shown, using hpr1Delta mutants, that the nascent mRNA can diminish transcription elongation efficiency and promote recombination and support a model to explain the connection between recombination, transcription, and mRNA metabolism.
Journal ArticleDOI
Human CtIP Mediates Cell Cycle Control of DNA End Resection and Double Strand Break Repair
Pablo Huertas,Stephen P. Jackson +1 more
TL;DR: It is established that Thr-847 mutations to either Ala or Glu affect DSB repair efficiency, cause hypersensitivity toward DSB-generating agents, and affect the frequency and nature of radiation-induced chromosomal rearrangements, suggesting that CDK-mediated control of resection in human cells operates by mechanisms similar to those recently established in yeast.
Journal ArticleDOI
CDK targets Sae2 to control DNA-end resection and homologous recombination.
Pablo Huertas,Felipe Cortés-Ledesma,Alessandro A. Sartori,Alessandro A. Sartori,Andrés Aguilera,Stephen P. Jackson +5 more
TL;DR: This work establishes that cell-cycle control of DSB resection in Saccharomyces cerevisiae results from the phosphorylation by CDK of an evolutionarily conserved motif in the Sae2 protein, and shows that mutating Ser’267 of Sae 2 to a non-phosphorylatable residue causes phenotypes comparable to those of a sae2Δ null mutant.
Journal ArticleDOI
DNA resection in eukaryotes: deciding how to fix the break
TL;DR: Recent findings on the mechanisms of resection in eukaryotes are reviewed, insights into the regulatory strategies that control it are provided, and the consequences of both its impairment and its deregulation are highlighted.
Journal ArticleDOI
BRCA1 Accelerates CtIP-Mediated DNA-End Resection
Andrés Cruz-García,Andrés Cruz-García,Ana López-Saavedra,Ana López-Saavedra,Pablo Huertas,Pablo Huertas +5 more
TL;DR: An assay is developed to study DNA resection in higher eukaryotes at high resolution and demonstrates that the BRCA1-CtIP interaction, albeit not essential for resection, modulates the speed at which this process takes place.