Cotranscriptionally Formed DNA:RNA Hybrids Mediate Transcription Elongation Impairment and Transcription-Associated Recombination
Pablo Huertas,Andrés Aguilera +1 more
Reads0
Chats0
TLDR
It is shown, using hpr1Delta mutants, that the nascent mRNA can diminish transcription elongation efficiency and promote recombination and support a model to explain the connection between recombination, transcription, and mRNA metabolism.About:
This article is published in Molecular Cell.The article was published on 2003-09-01 and is currently open access. It has received 677 citations till now. The article focuses on the topics: General transcription factor & RNA polymerase II.read more
Citations
More filters
Journal ArticleDOI
Causes and consequences of replication stress.
TL;DR: In this paper, the kinase ATR (ATM- and Rad3-related) stabilizes and helps to restart stalled replication forks, avoiding the generation of DNA damage and genome instability.
Journal ArticleDOI
Pre-mRNA processing reaches back to transcription and ahead to translation.
TL;DR: This review discusses how pre-mRNA processing, including 5' cap addition, splicing, and polyadenylation, contributes to both the efficiency and fidelity of gene expression.
Journal ArticleDOI
R Loops: From Transcription Byproducts to Threats to Genome Stability
TL;DR: The factors and cellular processes that control R loop formation and the mechanisms by which R loops may influence gene expression and the integrity of the genome are discussed.
Journal ArticleDOI
Genome instability: a mechanistic view of its causes and consequences
TL;DR: The causes and consequences of instability are reviewed with the aim of providing a mechanistic perspective on the origin of genomic instability.
Journal ArticleDOI
Exporting RNA from the nucleus to the cytoplasm
Alwin Köhler,Ed Hurt +1 more
TL;DR: The transport of RNA molecules from the nucleus to the cytoplasm is fundamental for gene expression and understanding the mechanisms that connect RNP formation with export is a major challenge in the field.
References
More filters
Journal ArticleDOI
DNA double-strand breaks: signaling, repair and the cancer connection.
TL;DR: Recent progress is described in understanding of how cells detect and signal the presence and repair of one particularly important form of DNA damage induced by ionizing radiation—the DNA double-strand break (DSB).
Journal ArticleDOI
Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds.
Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds (Saccharomyces cerevisiae; plasmid; multicopy vector; polylinker; promoter; heterologous expression; cDNA cloning)
TL;DR: In this paper, an expression system for Saccharomyces cerevisiae (Sc) is developed which allows the constitutive expression of proteins at different levels over a range of three orders of magnitude and in different genetic backgrounds.
Journal ArticleDOI
Elevated recombination rates in transcriptionally active DNA.
TL;DR: Northern analysis of strains containing plasmid inserts with various promoter mutations suggests that the stimulation in recombination is mediated by events initiating within the integrated plasmID sequences.
Journal ArticleDOI
Integrating mRNA Processing with Transcription
TL;DR: The phosphorylated CTD of RNA polymerase II provides key molecular contacts with these mRNA processing reactions throughout transcriptional elongation and termination.
Related Papers (5)
Inactivation of the SR Protein Splicing Factor ASF/SF2 Results in Genomic Instability
Xialu Li,James L. Manley +1 more