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Philippe Duchateau
Researcher at University of California, San Francisco
Publications - 75
Citations - 5540
Philippe Duchateau is an academic researcher from University of California, San Francisco. The author has contributed to research in topics: Genome engineering & Meganuclease. The author has an hindex of 35, co-authored 64 publications receiving 4955 citations. Previous affiliations of Philippe Duchateau include University of Copenhagen & Royal Holloway, University of London.
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Journal ArticleDOI
A combinatorial approach to create artificial homing endonucleases cleaving chosen sequences
Julianne Smith,Sylvestre Grizot,Sylvain Arnould,Aymeric Duclert,Jean-Charles Epinat,Patrick Chames,Jesús Prieto,Pilar Redondo,Francisco J. Blanco,Jerónimo Bravo,Guillermo Montoya,Frédéric Pâques,Philippe Duchateau +12 more
TL;DR: The first artificial HEs whose specificity has been entirely redesigned to cleave a naturally occurring sequence are described, demonstrating that the plasticity of LAGLIDADG endonucleases allows extensive engineering, and provide a general method to create novel endon nucleases with tailored specificities.
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Meganucleases and other tools for targeted genome engineering: perspectives and challenges for gene therapy.
George H. Silva,Laurent Poirot,Roman Galetto,Julianne Smith,Guillermo Montoya,Philippe Duchateau,Frédéric Paques +6 more
TL;DR: These alternative approaches based on non-viral vectorization and/or targeted insertion aimed at achieving safer gene transfer are reviewed, with a special emphasis on megan nucleases, a family of naturally occurring rare-cutting endonucleases, and speculate on their current and future potential.
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Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology
Fayza Daboussi,Sophie Leduc,Alan Maréchal,Gwendoline Dubois,Valérie Guyot,Christophe Perez-Michaut,Alberto Amato,Angela Falciatore,Angela Falciatore,Alexandre Juillerat,Marine Beurdeley,Daniel F. Voytas,Laurent Cavarec,Philippe Duchateau +13 more
TL;DR: The generation of an enhanced lipid-producing strain through the disruption of the UDP-glucose pyrophosphorylase gene exemplifies the power of genome engineering to harness diatoms for biofuel production.
Journal ArticleDOI
Meganucleases and DNA double-strand break-induced recombination : Perspectives for gene therapy
TL;DR: Current systems based on redesigned endonucleases will be presented, with a special emphasis on the recent advances in homing endonuclease engineering, and the main issues that will need to be addressed in order to bring this promising technology to the patient.
Journal ArticleDOI
Engineering of large numbers of highly specific homing endonucleases that induce recombination on novel DNA targets.
Sylvain Arnould,Patrick Chames,Christophe Perez,Emmanuel Lacroix,Aymeric Duclert,Jean-Charles Epinat,Francois Stricher,Anne-Sophie Petit,Amélie Patin,Sophie Guillier,Sandra Rolland,Jesús Prieto,Francisco J. Blanco,Jerónimo Bravo,Guillermo Montoya,Luis Serrano,Philippe Duchateau,Frédéric Pâques +17 more
TL;DR: It is shown that a semi-rational approach can be used to derive hundreds of novel proteins from I-CreI, a homing endonuclease from the LAGLIDADG family, and rules for protein/DNA interaction can be inferred from statistical analysis.