G
George H. Silva
Researcher at University of Giessen
Publications - 29
Citations - 1308
George H. Silva is an academic researcher from University of Giessen. The author has contributed to research in topics: Transcription activator-like effector nuclease & Genome engineering. The author has an hindex of 14, co-authored 29 publications receiving 1238 citations. Previous affiliations of George H. Silva include University of Copenhagen & University at Albany, SUNY.
Papers
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Journal ArticleDOI
Meganucleases and other tools for targeted genome engineering: perspectives and challenges for gene therapy.
George H. Silva,Laurent Poirot,Roman Galetto,Julianne Smith,Guillermo Montoya,Philippe Duchateau,Frédéric Paques +6 more
TL;DR: These alternative approaches based on non-viral vectorization and/or targeted insertion aimed at achieving safer gene transfer are reviewed, with a special emphasis on megan nucleases, a family of naturally occurring rare-cutting endonucleases, and speculate on their current and future potential.
Patent
Method for the generation of compact tale-nucleases and uses thereof
Claudia Bertonati,Philippe Duchateau,Epinat Jean-Charles,Alexandre Juillerat,George H. Silva,Julien Valton +5 more
TL;DR: In this paper, a method for the generation of compact Transcription Activator-Like Effector Nucleases (TALENs) that can efficiently target and process double-stranded DNA is presented.
Journal ArticleDOI
Compact designer TALENs for efficient genome engineering
Marine Beurdeley,Fabian Bietz,Jin Li,Séverine Thomas,Thomas Stoddard,Alexandre Juillerat,Feng Zhang,Daniel F. Voytas,Philippe Duchateau,George H. Silva +9 more
TL;DR: A significant improvement to the standard transcription activator-like effector nuclease architecture is reported by leveraging the partially specific I-TevI catalytic domain to create a new class of monomeric, DNA-cleaving enzymes.
Journal ArticleDOI
Crystal structure of the thermostable archaeal intron-encoded endonuclease I-DmoI
TL;DR: The overall fold of I-DmoI is similar to that of two other LAGLIDADG proteins for which the structures are known, I-CreI and the endonuclease domain of PI-SceI, but the loops connecting the beta-strands differ most in the loops connected to the respective DNA target site sizes and geometries.
Journal ArticleDOI
Comprehensive analysis of the specificity of transcription activator-like effector nucleases
Alexandre Juillerat,Gwendoline Dubois,Julien Valton,Séverine Thomas,Stefano Stella,Alan Maréchal,Stéphanie Langevin,Nassima Benomari,Claudia Bertonati,George H. Silva,Fayza Daboussi,Jean-Charles Epinat,Guillermo Montoya,Aymeric Duclert,Philippe Duchateau +14 more
TL;DR: It is reported that TALEN can only accommodate a relatively small number of position-dependent mismatches while maintaining a detectable activity at endogenous loci in vivo, demonstrating the high specificity of these molecular tools.