P
Pu Gao
Researcher at Chinese Academy of Sciences
Publications - 41
Citations - 3025
Pu Gao is an academic researcher from Chinese Academy of Sciences. The author has contributed to research in topics: Biology & Medicine. The author has an hindex of 17, co-authored 33 publications receiving 2010 citations. Previous affiliations of Pu Gao include Memorial Sloan Kettering Cancer Center & Rutgers University.
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Journal ArticleDOI
Cyclic [G(2′,5′)pA(3′,5′)p] Is the Metazoan Second Messenger Produced by DNA-Activated Cyclic GMP-AMP Synthase
Pu Gao,Manuel Ascano,Wu Yang,Winfried Barchet,Barbara L. Gaffney,Thomas Zillinger,Artem A. Serganov,Liu Yizhou,Roger A. Jones,Gunther Hartmann,Thomas Tuschl,Dinshaw J. Patel +11 more
TL;DR: Structural, chemical, biochemical, and cellular assays are combined to demonstrate that this second messenger contains G(2',5')pA and A(3',5']pG phosphodiester linkages, designated c[G(2,5')sDNA binding, cGAS] as a founding member of a family of metazoan 2',5'-containing cyclic heterodinucleotide second messengers distinct from bacterial 3',5' cyclic dinucleotides
Journal ArticleDOI
Structure-function analysis of STING activation by c[G(2',5')pA(3',5')p] and targeting by antiviral DMXAA.
Pu Gao,Manuel Ascano,Thomas Zillinger,Weiyi Wang,Peihong Dai,Artem A. Serganov,Barbara L. Gaffney,Stewart Shuman,Roger A. Jones,Liang Deng,Gunther Hartmann,Winfried Barchet,Thomas Tuschl,Dinshaw J. Patel +13 more
TL;DR: A unique point mutation (S162A) was identified within the cyclic-dinucleotide-binding site of hSTING that rendered it sensitive to the otherwise mouse-specific drug DMXAA, a conclusion validated by binding studies.
Journal ArticleDOI
PAM-Dependent Target DNA Recognition and Cleavage by C2c1 CRISPR-Cas Endonuclease.
TL;DR: Structural comparison of C2c1 ternary complexes with their Cas9 and Cpf1 counterparts highlights the diverse mechanisms adopted by these distinct CRISPR-Cas systems, thereby broadening and enhancing their applicability as genome editing tools.
Journal ArticleDOI
Small molecule inhibition of cGAS reduces interferon expression in primary macrophages from autoimmune mice.
Jessica Vincent,Carolina Adura,Pu Gao,Pu Gao,Antonio Luz,Antonio Luz,L. Lama,L. Lama,Asano Yasutomi,Rei Okamoto,Toshihiro Imaeda,Aida Jumpei,Katherine Rothamel,Tasos Gogakos,Tasos Gogakos,Joshua Steinberg,Joshua Steinberg,Seth A. Reasoner,Kazuyoshi Aso,Thomas Tuschl,Thomas Tuschl,Dinshaw J. Patel,J. Fraser Glickman,Manuel Ascano +23 more
TL;DR: It is found that a chemically improved member, RU.521, is active and selective in cellular assays of cyclic GMP-AMP synthase-mediated signaling and reduces constitutive expression of interferon in macrophages from a mouse model of Aicardi-Goutières syndrome.
Journal ArticleDOI
Type V CRISPR-Cas Cpf1 endonuclease employs a unique mechanism for crRNA-mediated target DNA recognition.
TL;DR: Structural comparison between the AsPf1-crRNA-DNA ternary complex and the recently reported Lachnospiraceae bacterium Cpf1 (LbCpf 1)-crRNA binary complex identifies a unique mechanism employed by CpF1 for target recognition.