Qiaolin Deng

TL;DR: Applying Smart-Seq to circulating tumor cells from melanomas, it is found that although gene expression estimates from single cells have increased noise, hundreds of differentially expressed genes could be identified using few cells per cell type.

TL;DR: It is concluded that independent and stochastic allelic transcription generates abundant random monoallelic expression in the mammalian cell.

TL;DR: A comprehensive transcriptional map of human embryo development, including the sequenced transcriptomes of 1,529 individual cells from 88 human preimplantation embryos, shows that cells undergo an intermediate state of co-expression of lineage-specific genes, followed by a concurrent establishment of the trophectoderm, epiblast, and primitive endoderm lineages, which coincide with blastocyst formation.

TL;DR: It is shown that the homeodomain proteins Lmx1a and Msx1 function as determinants of midbrain dopamine neurons, cells that degenerate in patients with Parkinson's disease, and suggest that they may be essential tools in cell replacement strategies in Parkinson’s disease.

TL;DR: This work generates electrophysiological and molecular profiles of 58 neocortical cells and shows that gene expression patterns can be used to infer the morphological and physiological properties such as axonal arborization and action potential amplitude of individual neurons.