R
Rajashree A. Deshpande
Researcher at University of Texas at Austin
Publications - 33
Citations - 1635
Rajashree A. Deshpande is an academic researcher from University of Texas at Austin. The author has contributed to research in topics: Homologous recombination & DNA. The author has an hindex of 17, co-authored 28 publications receiving 1371 citations. Previous affiliations of Rajashree A. Deshpande include National Chemical Laboratory & University of Michigan.
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Journal ArticleDOI
ATM activation in the presence of oxidative stress
TL;DR: It is shown that ATM activation by DSBs is inhibited in the presence of H2O2 because oxidation blocks the ability of MRN to bind to DNA, however, ATM activation via direct oxidation by H2 O2 complements the loss of MRn/DSB-dependent activation and contributes significantly to the overall level of ATM activity.
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Catalytic and Noncatalytic Roles of the CtIP Endonuclease in Double-Strand Break End Resection
Nodar Makharashvili,Anthony T. Tubbs,Soo Hyun Yang,Hailong Wang,Olivia Barton,Yi Zhou,Rajashree A. Deshpande,Ji-Hoon Lee,Markus Löbrich,Barry P. Sleckman,Xiaohua Wu,Tanya T. Paull +11 more
TL;DR: It is found that recombinant human CtIP exhibits 5' flap endonuclease activity on branched DNA structures, independent of the MRN complex, suggesting that the nucleaseActivity of CtIP is specifically required for the removal of DNA adducts at sites of DNA breaks.
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Mre11 Is Essential for the Removal of Lethal Topoisomerase 2 Covalent Cleavage Complexes
Nguyen Ngoc Hoa,Tsubasa Shimizu,Zhong-Wei Zhou,Zhao-Qi Wang,Rajashree A. Deshpande,Tanya T. Paull,Salma Akter,Masataka Tsuda,Ryohei Furuta,Ken Tsutsui,Shunichi Takeda,Hiroyuki Sasanuma +11 more
TL;DR: In this paper, the authors characterized Mre11-deficient (MRE11 −/− ) and nucleasedeficient MRE11 ( MRE 11 −/H129N ) chicken DT40 and human lymphoblast cell lines.
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Single-Molecule Imaging Reveals How Mre11-Rad50-Nbs1 Initiates DNA Break Repair
Logan R. Myler,Ignacio F. Gallardo,Michael M. Soniat,Rajashree A. Deshpande,Xenia B. Gonzalez,Yoori Kim,Tanya T. Paull,Ilya J. Finkelstein +7 more
TL;DR: High-throughput single-molecule microscopy is used to show that MRN searches for free DNA ends by one-dimensional facilitated diffusion, even on nucleosome-coated DNA, providing a mechanism for how MRN promotes homologous recombination on nucleOSome- coated DNA.
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ATP-driven Rad50 conformations regulate DNA tethering, end resection, and ATM checkpoint signaling.
Rajashree A. Deshpande,Gareth J. Williams,Oliver Limbo,R. Scott Williams,Jeff Kuhnlein,Ji-Hoon Lee,Scott Classen,Grant Guenther,Paul Russell,John A. Tainer,John A. Tainer,Tanya T. Paull +11 more
TL;DR: Crystal structures, X‐ray scattering, biochemical assays, and functional analyses of mutant PfRad50 complexes show that the ATP‐induced ‘closed’ conformation promotes DNA end binding and end tethering, while hydrolysis‐induced opening is essential for DNA resection.