Rebecca J. Dearman

TL;DR: An overview of the phenomenon of protein aggregation, the production of unwanted aggregates during bioprocessing, and how the immune response to aggregated protein differs from that provoked by non-aggregated protein is provided.

TL;DR: A database of robust in vivo data to calibrate, evaluate, and eventually validate new approaches for skin sensitization testing is provided, which represents both the chemical and biologic diversity that is known to exist for chemical allergens and non‐allergens.

TL;DR: It was found that like anti‐TNF‐α, anti‐IL‐1β administered systemically to mice (by intraperitoneal injection), prior to skin sensitization with the contact allergen oxazolone, resulted in a marked inhibition of DC accumulation in draining lymph nodes.

TL;DR: Overall, assay pH did not influence the time to disappearance of the full-length protein or protein fragments, however, results across laboratories were more consistent at pH 1.2 than pH 2.0, demonstrating that this common protocol for evaluating the in vitro digestibility of proteins is reproducible and yields consistent results when performed using the same proteins at different laboratories.

TL;DR: This work considers the potential contribution that individual epitopes may make to the allergenicity of a protein, and considers the effects that resistance to proteolysis, post-translational glycosylation, and enzymatic activity may have.