R
Renaud Wagner
Researcher at University of Strasbourg
Publications - 44
Citations - 1227
Renaud Wagner is an academic researcher from University of Strasbourg. The author has contributed to research in topics: Pichia pastoris & G protein-coupled receptor. The author has an hindex of 18, co-authored 39 publications receiving 1101 citations. Previous affiliations of Renaud Wagner include Centre national de la recherche scientifique & Louis Pasteur University.
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Journal ArticleDOI
Enhancing functional production of G protein-coupled receptors in Pichia pastoris to levels required for structural studies via a single expression screen
Nicolas André,Nadia Cherouati,Cécile Prual,Tania Steffan,Gabrielle Zeder-Lutz,Thierry Magnin,Franc Pattus,Hartmut Michel,Renaud Wagner,Christoph Reinhart +9 more
TL;DR: Overall, the number of GPCR targets that can be produced in the authors' laboratories in sufficient amounts for structural studies has more than doubled, and a general approach for increasing yields of functional mammalian GPCRs severalfold over standard expression conditions is suggested.
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Structural genomics on membrane proteins: comparison of more than 100 GPCRs in 3 expression systems.
Kenneth Lundstrom,Renaud Wagner,Christoph Reinhart,Aline Desmyter,Nadia Cherouati,Thierry Magnin,Gabrielle Zeder-Lutz,Mélanie Courtot,Cécile Prual,Nicolas André,Ghérici Hassaine,Hartmut Michel,Christian Cambillau,Franc Pattus +13 more
TL;DR: The MePNet (Membrane Protein Network) was established to overexpress a large number of GPCRs in three major expression systems, based on Escherichia coli, Pichia pastoris and Semliki Forest virus vectors, and functional activity was determined by binding assays in yeast and mammalian cells.
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Detergent-free Isolation of Functional G Protein-Coupled Receptors into Nanometric Lipid Particles
Christel Logez,Marjorie Damian,Céline Legros,Clémence Dupré,Mélody Guéry,Sophie Mary,Renaud Wagner,Céline M'Kadmi,Olivier Nosjean,Benjamin Fould,Jacky Marie,Jean-Alain Fehrentz,Jean Martinez,Gilles Ferry,Jean A. Boutin,Jean-Louis Banères +15 more
TL;DR: The potential of styrene-maleic stabilized lipid discs for analyzing the molecular bases of GPCR-mediated signaling in a well-controlled membrane-like environment is highlighted.
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Semliki Forest virus vectors for overexpression of 101 G protein-coupled receptors in mammalian host cells
Ghérici Hassaine,Renaud Wagner,Juliette Kempf,Nadja Cherouati,Nhouzha Hassaine,Cécile Prual,Nicolas André,Christoph Reinhart,Franc Pattus,Kenneth Lundstrom +9 more
TL;DR: Semliki Forest virus vectors were applied for the evaluation of 101 G protein-coupled receptors in three mammalian cell lines and 95 of the 101 tested GPCRs showed positive signals, suggesting receptor yields in the range of 1 mg/L or higher, suitable for structural biology applications.
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Quantification of Detergents Complexed with Membrane Proteins
Vincent Chaptal,Frédéric Delolme,Arnaud Kilburg,Sandrine Magnard,Cédric Montigny,Martin Picard,Charlène Prier,Luca Monticelli,Olivier Bornert,Morgane Agez,Stéphanie Ravaud,Cédric Orelle,Renaud Wagner,Anass Jawhari,Isabelle Broutin,Eva Pebay-Peyroula,Jean-Michel Jault,H. Ronald Kaback,Marc le Maire,Pierre Falson +19 more
TL;DR: A method based on matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) that allows quantification of pure or mixed detergents in complex with membrane proteins, and validated the method with a wide variety of deterGents and membrane proteins.