R
Robert H. Shoemaker
Researcher at National Institutes of Health
Publications - 216
Citations - 23122
Robert H. Shoemaker is an academic researcher from National Institutes of Health. The author has contributed to research in topics: Cancer & DNA damage. The author has an hindex of 55, co-authored 197 publications receiving 21697 citations. Previous affiliations of Robert H. Shoemaker include University of Bologna & Strong Memorial Hospital.
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Journal Article
Feasibility of Drug Screening with Panels of Human Tumor Cell Lines Using a Microculture Tetrazolium Assay
Michael C. Alley,Dominic A. Scudiero,Anne Monks,Miriam L. Hursey,Maciej Czerwinski,Donald L. Fine,B. J. Abbott,Joseph G. Mayo,Robert H. Shoemaker,Michael R. Boyd +9 more
TL;DR: Since the microculture tetrazolium assay provides sensitive and reproducible indices of growth as well as drug sensitivity in individual cell lines over the course of multiple passages and several months' cultivation, it appears suitable for initial-stage in vitro drug screening.
Journal ArticleDOI
Feasibility of a High-Flux Anticancer Drug Screen Using a Diverse Panel of Cultured Human Tumor Cell Lines
Anne Monks,Dominic A. Scudiero,Philip Skehan,Robert H. Shoemaker,Kenneth D. Paull,David T. Vistica,Curtis Hose,John Langley,Paul Cronise,Anne Vaigro-Wolff,Marcia Gray-Goodrich,H. D. Campbell,Joseph G. Mayo,Michael R. Boyd +13 more
TL;DR: A pilot-scale, in vitro, anticancer drug screen utilizing a panel of 60 human tumor cell lines organized into subpanels representing leukemia, melanoma, and cancers of the lung, colon, kidney, ovary, and central nervous system is described.
Journal Article
Evaluation of a Soluble Tetrazolium/Formazan Assay for Cell Growth and Drug Sensitivity in Culture Using Human and Other Tumor Cell Lines
Dominic A. Scudiero,Robert H. Shoemaker,Kenneth D. Paull,Anne Monks,Siobhan Tierney,Thomas H. Nofziger,Michael J. Currens,Donna Seniff,Michael R. Boyd +8 more
TL;DR: The new XTT reagent provides for a simplified, in vitro cell growth assay with possible applicability to a variety of problems in cellular pharmacology and biology, but still shares many of the limitations and potential pitfalls of MTT or other tetrazolium-based assays.
Journal ArticleDOI
The NCI60 human tumour cell line anticancer drug screen
TL;DR: The development, use and productivity of the NCI60 screen are reviewed, highlighting several outcomes that have contributed to advances in cancer chemotherapy.
Journal ArticleDOI
Comparison of In Vitro Anticancer-Drug-Screening Data Generated With a Tetrazolium Assay Versus a Protein Assay Against a Diverse Panel of Human Tumor Cell Lines
Larry Rubinstein,Robert H. Shoemaker,Kenneth D. Paull,Richard M. Simon,S. Tosini,Philip Skehan,Dominic A. Scudiero,Anne Monks,Michael R. Boyd +8 more
TL;DR: A detailed comparison of data generated by each type of assay was undertaken, and results indicate that under the experimental conditions used and within the limits of the data analyses, the assays perform similarly.