Robert Sarfati

TL;DR: 3'-substituted-2'-deoxyribonucleotide-5'-triphosphates corresponding to A, T, G and C are synthesized, giving specific fluorescent properties to each nucleotide (nt).

TL;DR: Results demonstrate that both fragments of the 43-kDa form of adenylate cyclase are essential for a high level of enzymatic activity.

TL;DR: A truncated Bordetella pertussis cya gene product was expressed in Escherichia coli and purified by affinity chromatography on calmodulin-agarose, suggesting that interactive contacts between ordered portions of T28 and T19 in the intact protein participate in their own stabilization and in stabilization of the whole tertiary structure.

TL;DR: Substrate specificity studies showed that CMP kinase from E. coli is active with ATP, dATP, or GTP as donors and with CMP, dCMP, and arabinofuranosyl-CMP as acceptors, and sequence comparison suggested conservation of the global fold found in adenylate kinases or in several CMP/UMP kinases.

TL;DR: Norleucine-substituted adenylate kinase shows structural and catalytic properties similar to the wild-type protein as indicated by circular dichroism spectroscopy and kinetic experiments but exhibits a much higher resistance to hydrogen peroxide inactivation under denaturing conditions.