Characterization of the calmodulin-binding and of the catalytic domains of Bordetella pertussis adenylate cyclase.
TLDR
Results demonstrate that both fragments of the 43-kDa form of adenylate cyclase are essential for a high level of enzymatic activity.About:
This article is published in Journal of Biological Chemistry.The article was published on 1989-03-05 and is currently open access. It has received 99 citations till now. The article focuses on the topics: Cyclase & Bordetella pertussis.read more
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A bacterial two-hybrid system based on a reconstituted signal transduction pathway
TL;DR: In this genetic test, the involvement of a signaling cascade offers the unique property that association between the hybrid proteins can be spatially separated from the transcriptional activation readout, allowing a versatile design of screening procedures either for ligands that bind to a given "bait," as in the classical yeast two-hybrid system, or for molecules or mutations that block a given interaction between two proteins of interest.
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Sequence motifs for calmodulin recognition.
Allen R. Rhoads,Felix Friedberg +1 more
TL;DR: Three recognition motifs for CaM interaction are discussed in the context of experimental investigations of a variety of CaM target proteins and a modified version of the IQ motif as a consensus for Ca2+‐independent binding and two related motifs, termed 18‐14 and 1‐5‐10 based on the position of conserved hydrophobic residues, are proposed.
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Translocation of a hybrid YopE-adenylate cyclase from Yersinia enterocolitica into HeLa cells
Marie-Paule Sory,Guy R. Cornelis +1 more
TL;DR: The results demonstrate that Y. enterocolitica is able to transfer hybrid proteins into eukaryotic cells and can be used not only to study the mechanism of YopE translocation but also the fate of the other Yops or even of proteins secreted by other bacterial pathogens.
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Cyclic AMP in prokaryotes.
J. L. Botsford,J G Harman +1 more
TL;DR: This review summarizes recent work focused on elucidating the molecular mechanisms of CRP-cAMP complex-mediated processes in enteric coliforms and suggests a role for cAMP has been suggested in nitrogen fixation.
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The bacterial two-hybrid system based on adenylate cyclase reconstitution in Escherichia coli.
TL;DR: This review aims at giving the reader clear and most importantly simple instructions that should break any reticence to try the bacterial two-hybrid system, and exposing the advantages and disadvantages of the technique, and reviewing its diverse applications in the literature, which should help in deciding if it is the appropriate method to choose for the case at hand.
References
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
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Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins.
TL;DR: The algorithm is shown to be at least as good as, and usually superior to, the reported prediction methods assessed in the same way and the implication in protein folding is discussed.
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A modified spectrophotometric determination of chymotrypsin, trypsin, and thrombin.
TL;DR: The spectrophotometric procedure proposed by Schwert and Takenaka has been modified and extended to include the application to N-benzoyl-L-tyrosine ethyl ester and α-p-toluenesulphonyl- L-arginine methyl ester, allowing the determination of traces of chymotrypsin in the presence of relatively large amounts of trypsin.
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A simple method for the preparation of 32P-labelled adenosine triphosphate of high specific activity
IM Glynn,JB Chappell +1 more
TL;DR: Hydrolysis of the labeled ATP with heavy meromyosin indicates that 98 to 99% of the /sup 32/P is in the 1 -phosphate group.
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Disc electrophoresis in polyacrylamide gels: extension to new conditions of ph and buffer.
TL;DR: The purpose of this paper is to present a strategy that is useful in extending disc electrophoresis to new conditions of pH and constituent with considerably greater convenience than by using the rather complex calculations from the theory presented by Ornstein.