R
Robert Shroff
Researcher at National Institutes of Health
Publications - 7
Citations - 1700
Robert Shroff is an academic researcher from National Institutes of Health. The author has contributed to research in topics: Homologous recombination & Saccharomyces cerevisiae. The author has an hindex of 6, co-authored 7 publications receiving 1640 citations.
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Journal ArticleDOI
DNA Damage Response Pathway Uses Histone Modification to Assemble a Double-Strand Break-Specific Cohesin Domain
Elçin Ünal,Ayelet Arbel-Eden,Ulrike Sattler,Robert Shroff,Michael Lichten,James E. Haber,Douglas Koshland +6 more
TL;DR: It is shown in budding yeast that a single DSB induces the formation of a approximately 100 kb cohesin domain around the lesion, and analyses suggest that the primary DNA damage checkpoint kinases Mec1p and Tel1p phosphorylate histone H2AX to generate a large domain, which is permissive for cohesIn binding.
Journal ArticleDOI
Distribution and Dynamics of Chromatin Modification Induced by a Defined DNA Double-Strand Break
Robert Shroff,Ayelet Arbel-Eden,Duane R. Pilch,Grzegorz Ira,William M. Bonner,John H.J. Petrini,James E. Haber,Michael Lichten +7 more
TL;DR: This work reports the first molecular-level characterization of regional chromatin changes that accompany a DSB formed by the HO endonuclease in Saccharomyces cerevisiae, and indicates that localization of repair proteins to breaks is not likely to be the main function of this histone modification.
Journal ArticleDOI
Global mapping of meiotic recombination hotspots and coldspots in the yeast Saccharomyces cerevisiae
Jennifer L. Gerton,Joseph L. DeRisi,Robert Shroff,Michael Lichten,Patrick O. Brown,Thomas D. Petes +5 more
TL;DR: This work used DNA microarrays to estimate variation in the level of nearby meiotic DSBs for all 6,200 yeast genes and found hotspots and coldspots were nonrandomly associated with regions of high G + C base composition and certain transcriptional profiles.
Book ChapterDOI
Techniques for gamma-H2AX detection.
Asako J. Nakamura,Olga A. Sedelnikova,Christophe E. Redon,Duane R. Pilch,Natasha I Sinogeeva,Robert Shroff,Michael Lichten,William M. Bonner +7 more
TL;DR: This chapter presents techniques for revealing gamma-H2AX foci in cultured cells, in metaphase spreads from cultured Cells, in tissues, and in yeast.
Journal ArticleDOI
Characterization of the Meiosis-Specific Recombinase Dmc1 of Pneumocystis
Geetha Kutty,Guillaume Achaz,Frank Maldarelli,Ashok Varma,Robert Shroff,Steven Becker,Giovanna Fantoni,Joseph A. Kovacs +7 more
TL;DR: In this paper, an orthologue of dmc1, a gene specific for meiotic recombination in yeast, was identified and characterized in three species of Pneumocystis.