R
Rohith N. Krishna
Researcher at University of California, Berkeley
Publications - 12
Citations - 141
Rohith N. Krishna is an academic researcher from University of California, Berkeley. The author has contributed to research in topics: Pseudomonas putida & Chemistry. The author has an hindex of 5, co-authored 9 publications receiving 88 citations. Previous affiliations of Rohith N. Krishna include Lawrence Berkeley National Laboratory & Joint BioEnergy Institute.
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Journal ArticleDOI
Massively Parallel Fitness Profiling Reveals Multiple Novel Enzymes in Pseudomonas putida Lysine Metabolism.
Mitchell G. Thompson,Mitchell G. Thompson,Mitchell G. Thompson,Jacquelyn M. Blake-Hedges,Jacquelyn M. Blake-Hedges,Jacquelyn M. Blake-Hedges,Pablo Cruz-Morales,Pablo Cruz-Morales,Pablo Cruz-Morales,Jesus F. Barajas,Samuel C. Curran,Samuel C. Curran,Samuel C. Curran,Christopher B. Eiben,Christopher B. Eiben,Christopher B. Eiben,Nicholas C. Harris,Veronica T. Benites,Veronica T. Benites,Jennifer W. Gin,Jennifer W. Gin,William A. Sharpless,William A. Sharpless,William A. Sharpless,Frederick F. Twigg,Will Skyrud,Rohith N. Krishna,Rohith N. Krishna,Rohith N. Krishna,Jose Henrique Pereira,Jose Henrique Pereira,Edward E. K. Baidoo,Edward E. K. Baidoo,Christopher J. Petzold,Christopher J. Petzold,Paul D. Adams,Paul D. Adams,Paul D. Adams,Adam P. Arkin,Adam P. Arkin,Adam M. Deutschbauer,Adam M. Deutschbauer,Jay D. Keasling +42 more
TL;DR: The utility of RB-TnSeq for discovering novel metabolic pathways in even well-studied bacteria, as well as its utility a powerful tool for validating previous research, demonstrated that biological novelty can be rapidly identified using unbiased experimental genetics and that RB-NSeq can be used to rapidly validate previous results.
Journal ArticleDOI
Fatty Acid and Alcohol Metabolism in Pseudomonas putida: Functional Analysis Using Random Barcode Transposon Sequencing.
Mitchell G. Thompson,Matthew R. Incha,Matthew R. Incha,Matthew R. Incha,Allison N. Pearson,Allison N. Pearson,Allison N. Pearson,M. Schmidt,M. Schmidt,M. Schmidt,William A. Sharpless,William A. Sharpless,Christopher B. Eiben,Christopher B. Eiben,Christopher B. Eiben,Pablo Cruz-Morales,Pablo Cruz-Morales,Pablo Cruz-Morales,Jacquelyn M. Blake-Hedges,Jacquelyn M. Blake-Hedges,Jacquelyn M. Blake-Hedges,Yuzhong Liu,Yuzhong Liu,Catharine A. Adams,Robert W. Haushalter,Robert W. Haushalter,Rohith N. Krishna,Rohith N. Krishna,Patrick Lichtner,Patrick Lichtner,Lars M. Blank,Aindrila Mukhopadhyay,Aindrila Mukhopadhyay,Adam M. Deutschbauer,Patrick M. Shih,Jay D. Keasling +35 more
TL;DR: Functional evidence for the putative roles of hundreds of genes involved in the fatty acid and alcohol metabolism of the Pseudomonas putida bacterium is provided, providing a framework facilitating precise genetic changes to prevent product degradation and to channel the flux of specific pathway intermediates as desired.
Journal ArticleDOI
Robust Characterization of Two Distinct Glutarate Sensing Transcription Factors of Pseudomonas putida l-Lysine Metabolism.
Mitchell G. Thompson,Mitchell G. Thompson,Mitchell G. Thompson,Zak Costello,Zak Costello,Niklas F. C. Hummel,Niklas F. C. Hummel,Pablo Cruz-Morales,Pablo Cruz-Morales,Pablo Cruz-Morales,Jacquelyn M. Blake-Hedges,Jacquelyn M. Blake-Hedges,Jacquelyn M. Blake-Hedges,Rohith N. Krishna,Rohith N. Krishna,Rohith N. Krishna,Will Skyrud,Allison N. Pearson,Allison N. Pearson,Matthew R. Incha,Matthew R. Incha,Matthew R. Incha,Patrick M. Shih,Patrick M. Shih,Hector Garcia-Martin,Jay D. Keasling +25 more
TL;DR: A novel mathematical approach is developed to describe the usable range of detection for genetically encoded biosensors, which may be broadly useful in future efforts to better characterize biosensor performance.
Posted ContentDOI
Massively parallel fitness profiling reveals multiple novel enzymes in Pseudomonas putida lysine metabolism
Mitchell G. Thompson,Jacquelyn M. Blake-Hedges,Pablo Cruz-Morales,Jesus F. Barajas,Samuel C. Curran,Nicholas C. Harris,Veronica T. Benites,Jennifer W. Gin,Christopher B. Eiben,William A. Sharpless,Rohith N. Krishna,Edward E. K. Baidoo,Christopher J. Petzold,Adam P. Arkin,Adam M. Deutschbauer,Jay D. Keasling +15 more
TL;DR: Random Barcode Transposon Sequencing (RB-TnSeq), a genome-wide assay measuring the fitness of thousands of genes in parallel, is used to identify multiple novel enzymes in both L- and D-lysine metabolism, providing the first biochemical evidence of a bacterial 2OA catabolic pathway to central metabolites.
Journal ArticleDOI
Structural Mechanism of Regioselectivity in an Unusual Bacterial Acyl-CoA Dehydrogenase
Jacquelyn M. Blake-Hedges,Jacquelyn M. Blake-Hedges,Jacquelyn M. Blake-Hedges,Jose Henrique Pereira,Jose Henrique Pereira,Pablo Cruz-Morales,Pablo Cruz-Morales,Mitchell G. Thompson,Mitchell G. Thompson,Mitchell G. Thompson,Jesus F. Barajas,Jesus F. Barajas,Jesus F. Barajas,Jeffrey Chen,Rohith N. Krishna,Leanne Jade G. Chan,Leanne Jade G. Chan,Danika Nimlos,Catalina Alonso-Martinez,Edward E. K. Baidoo,Edward E. K. Baidoo,Yan Chen,Yan Chen,Jennifer W. Gin,Jennifer W. Gin,Leonard Katz,Leonard Katz,Christopher J. Petzold,Christopher J. Petzold,Paul D. Adams,Paul D. Adams,Jay D. Keasling +31 more
TL;DR: Biochemical and structural analysis is used to understand the mechanism of terminal alkene formation catalyzed by an γ,ẟ-ACAD from the biosynthesis of the polyketide natural product FK506, TcsD and predicts that a phylogenetically distant homolog of TCSD also regioselectively oxidizes α,β-unsaturated substrates.