Rosemary Kiernan

TL;DR: This work shows that the p65 subunit of NF-κB is acetylated by both p300 and PCAF on lysines 122 and 123 and proposes that acetylation of p65 plays a key role in IκΒα-mediated attenuation ofNF-κΓ transcriptional activity which is an important process that restores the latent state in post-induced cells.

TL;DR: It is shown that p300 and PCAF also directly acetylate Tat, suggesting that acetylation of Tat regulates two discrete and functionally critical steps in transcription, binding to an RNAP II CTD‐kinase and release of Tat from TAR RNA.

TL;DR: The purified HIV-1 Tat-associated factors from HeLa nuclear extract are purified and show that Tat forms two distinct and stable complexes, Tatcom1 and Tatcom2, which are important for P-TEFb function.

TL;DR: It is shown that Suv39H1, HP1γ and histone H3Lys9 trimethylation play a major role in chromatin‐mediated repression of integrated HIV‐1 gene expression and that HIV‐ 1 reactivation could be achieved after HP 1γ RNA interference.

TL;DR: It is shown that the proto-oncoprotein Hdm2 interacts with Tat and mediates its ubiquitination in vitro and in vivo, supporting the notion that ubiquitin has a non-proteolytic function in Tat-mediated transactivation.