Saheed Imam

TL;DR: It is shown that Gram-positive bacteria contain a highly diverse set of type IV pili, and PilFind, a rule-based algorithm for genome-wide identification of otherwise poorly conserved type IV pilins in any species, regardless of their association with TFP biosynthetic operons, can be an invaluable tool to study bacterial cellular processes known to involve typeIV pilus-like structures.

TL;DR: It is determined that, in addition to disrupting preflagellin processing, deleting pibD, which encodes the preflagllin peptidase, prevents the maturation of other H. volcanii type IV pilin-like proteins, indicating that a nonflageLLar typeIV pilus-like structure plays a critical role in H.volcANIi surface adhesion.

TL;DR: Evaluation of photoheterotrophic H2 production using iRsp1095 indicated that maximal yield would be obtained from growing cells, with this predicted maximum ~50% higher than that observed experimentally from wild type cells, and competing pathways that might prevent the achievement of this theoretical maximum were identified to guide future genetic studies.

TL;DR: iCre1355 represents an experimentally validated genome-scale reconstruction of C. reinhardtii metabolism that should serve as a useful resource for studying the metabolic processes of this and related microalgae.

TL;DR: A comprehensive mechanistic model of the TRN controlling the transition from N starvation to lipid accumulation is uncovered and generated predictive tools that will aid in devising strategies for the rational manipulation of regulatory and metabolic networks for better biofuel and biomass production.