Saima M. Sidik

TL;DR: In this article, the authors adapted CRISPR/Cas9 to assess the contribution of each gene from the parasite Toxoplasma gondii during infection of human fibroblasts.

TL;DR: The first genome-wide genetic screen of an apicomplexan parasite is presented, revealing essential functions during infection of human cells and providing broad-based functional information on T. gondii genes that will facilitate future approaches to expand the horizon of antiparasitic interventions.

TL;DR: In this article, the authors developed a rapid response, fully synthetic, single-dose, adjuvant-free dendrimer nanoparticle vaccine platform wherein antigens are encoded by encapsulated mRNA replicons.

TL;DR: The RNA-guided Cas9 nuclease is used to efficiently generate knockouts without selection, and to introduce point mutations and epitope tags into the T. gondii genome, which will streamline the functional analysis of parasite genes and enable high-throughput engineering of their genomes.

TL;DR: GRA17 and GRA23 provide a molecular basis for PVM permeability and nutrient access and are identified as a Toxoplasma-secreted protein that localizes to the parasitophorous vacuole membrane (PVM) and mediates passive transport of small molecules across the PVM.