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Sarah M. Williams
Researcher at Environmental Molecular Sciences Laboratory
Publications - 17
Citations - 403
Sarah M. Williams is an academic researcher from Environmental Molecular Sciences Laboratory. The author has contributed to research in topics: Proteomics & Proteome. The author has an hindex of 5, co-authored 8 publications receiving 132 citations.
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Journal ArticleDOI
An Improved Boosting to Amplify Signal with Isobaric Labeling (iBASIL) Strategy for Precise Quantitative Single-cell Proteomics.
Chia-Feng Tsai,Rui Zhao,Sarah M. Williams,Ronald J. Moore,Kendall Schultz,William B. Chrisler,Ljiljana Paša-Tolić,Karin D. Rodland,Richard D. Smith,Tujin Shi,Ying Zhu,Tao Liu +11 more
TL;DR: The improved Boosting to Amplify Signal with Isobaric Labeling (iBASIL) strategy allows for precise and robust quantitative single-cell proteomics, and highlights the importance of carefully evaluating and optimizing the boosting ratios and MS data acquisition conditions for achieving robust, comprehensive proteomic analysis of single cells.
Journal ArticleDOI
Automated Coupling of Nanodroplet Sample Preparation with Liquid Chromatography–Mass Spectrometry for High-Throughput Single-Cell Proteomics
Sarah M. Williams,Andrey V. Liyu,Chia-Feng Tsai,Ronald J. Moore,Daniel J. Orton,William B. Chrisler,Matthew J. Gaffrey,Tao Liu,Richard D. Smith,Ryan T. Kelly,Ryan T. Kelly,Ljiljana Paša-Tolić,Ying Zhu +12 more
TL;DR: A nanoPOTS autosampler allowing fully automated sample injection from nanowells to LC-MS systems and a sample drying, extraction, and loading workflow to enable reproducible and reliable sample injection are developed.
Journal ArticleDOI
High-throughput and high-efficiency sample preparation for single-cell proteomics using a nested nanowell chip.
Jongmin Woo,Sarah M. Williams,Lye Meng Markillie,Song Feng,Chai-Feng Tsai,Victor Aguilera-Vazquez,Ryan L. Sontag,Ronald J. Moore,Dehong Hu,Hardeep S. Mehta,Joshua Cantlon-Bruce,Tao Liu,Joshua N. Adkins,Richard D. Smith,Geremy Clair,Ljiljana Paša-Tolić,Ying Zhu +16 more
TL;DR: In this paper, a nested nanoPOTS (N2) chip is proposed to improve protein recovery, operation robustness, and processing throughput for isobaric-labeling-based scProteomics workflow.
Journal ArticleDOI
Picoflow Liquid Chromatography–Mass Spectrometry for Ultrasensitive Bottom-Up Proteomics Using 2-μm-i.d. Open Tubular Columns
Piliang Xiang,Ying Zhu,Yu Yang,Zhitao Zhao,Sarah M. Williams,Ronald J. Moore,Ryan T. Kelly,Ryan T. Kelly,Richard D. Smith,Shaorong Liu +9 more
TL;DR: PicoLC-MS extends the limit of separation science and is expected to be a powerful tool for single cell proteomics.
Journal ArticleDOI
Sensitive Top-Down Proteomics Analysis of a Low Number of Mammalian Cells Using a Nanodroplet Sample Processing Platform.
Mowei Zhou,Naomi Uwugiaren,Sarah M. Williams,Ronald J. Moore,Rui Zhao,David R. Goodlett,David R. Goodlett,Irena Dapic,Ljiljana Paša-Tolić,Ying Zhu +9 more
TL;DR: It is hypothesized that the DDM detergent improves protein recovery by efficiently reducing nonspecific adsorption of intact proteins on container surfaces, while urea serves as a strong denaturant to disrupt noncovalent complexes and release intact proteins for downstream analysis.