Scott James Harrison

TL;DR: The development and successful application of a multiplex CRISPR/Cas9 system for genome engineering of up to 5 different genomic loci in one transformation step in baker's yeast Saccharomyces cerevisiae is reported.

TL;DR: Analysis of the 13C-labeled products revealed that the pathways of valine catabolism and leucine biosynthesis share a common pool of α-ketoisovalerate, and any single isozyme of pyruvate decarboxylase is sufficient for the formation of isobutyl alcohol from valine.

TL;DR: The metabolism of leucine to isoamyl alcohol in yeast was examined by 13C nuclear magnetic resonance spectroscopy and it was found that a pyruvate decarboxylase-like enzyme encoded byYDL080c appears to be the major route of decar boxylation of α-ketoisocaproate to iso amyl alcohol although disruption of this gene reveals that at least one other unidentified decarboxesize can substitute to a minor extent.

TL;DR: Results suggest that metabolomic studies can be used to ascertain metabolic signatures of disease in a non-invasive fashion and should provide insight into aberrant biochemical pathways and may provide diagnostic markers and targets for drug design.

TL;DR: The metabolism of isoleucine to active amyl alcohol in yeast was examined by the use of13C nuclear magnetic resonance spectroscopy, combined gas chromatography-mass spectrometry, and a variety of mutants to show that the decarboxylation steps for each α-keto acid in the catabolic pathways of leucine, valine, and isolesucine are accomplished in subtly different ways.