Sean A. McKinney

TL;DR: The results strongly support a hand-over-hand model of motility, not an inchworm model, which moves processively on actin.

TL;DR: An analysis scheme is developed that casts single-molecule time-binned FRET trajectories as hidden Markov processes, allowing one to determine, based on probability alone, the most likely FRET-value distributions of states and their interconversion rates while simultaneously determining the mostlikely time sequence of underlying states for each trajectory.

TL;DR: Trolox in combination with the enzymatic oxygen-scavenging system eliminates Cy5 blinking, dramatically reduces photobleaching and improves the signal linearity at high excitation rates, significantly extending the applicability of single-molecule fluorescence techniques.

TL;DR: Relative rates indicate that multiple conformer transitions occur at each intermediate step of branch migration, allowing the junction to reach conformational equilibrium, providing a mechanism whereby the sequence-dependent conformational bias could determine the extent of genetic exchange upon junction resolution.

TL;DR: Using single-molecule fluorescence assays and hidden Markov modeling, the most direct evidence is shown that a RecA filament grows and shrinks primarily one monomer at a time and only at the extremities, supporting the proposal for a passive role of RecA-loading machineries in SSB removal.