Real-time observation of RecA filament dynamics with single monomer resolution.
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TLDR
Using single-molecule fluorescence assays and hidden Markov modeling, the most direct evidence is shown that a RecA filament grows and shrinks primarily one monomer at a time and only at the extremities, supporting the proposal for a passive role of RecA-loading machineries in SSB removal.About:
This article is published in Cell.The article was published on 2006-08-11 and is currently open access. It has received 289 citations till now. The article focuses on the topics: Protein filament.read more
Citations
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A practical guide to single-molecule FRET
TL;DR: A practical guide to using smFRET, focusing on the study of immobilized molecules that allow measurements of single-molecule reaction trajectories from 1 ms to many minutes, is provided.
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An Oxygen Scavenging System for Improvement of Dye Stability in Single-Molecule Fluorescence Experiments
TL;DR: An enzymatic oxygen scavenging system for improved dye stability in single-molecule experiments and it is found that biological reducing agents increase both the frequency and duration of blinking events of Cy5, an effect that scales with reducing potential.
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Analysis of Single-Molecule FRET Trajectories Using Hidden Markov Modeling
TL;DR: An analysis scheme is developed that casts single-molecule time-binned FRET trajectories as hidden Markov processes, allowing one to determine, based on probability alone, the most likely FRET-value distributions of states and their interconversion rates while simultaneously determining the mostlikely time sequence of underlying states for each trajectory.
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Advances in Single-Molecule Fluorescence Methods for Molecular Biology
TL;DR: In this exciting era of transitioning from in vitro to in vivo and in situ conditions, it is anticipated that SM fluorescence methodology will become a common tool of molecular biology.
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Mechanism of homologous recombination from the RecA-ssDNA/dsDNA structures.
TL;DR: It is shown that ssDNA and ATP bind to RecA–RecA interfaces cooperatively, explaining the ATP dependency of DNA binding, and the underwound, stretched filament conformation probably evolved to destabilize the donor duplex, freeing the complementary strand for homology sampling.
References
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TL;DR: The progress in applying single-molecule detection and single-Molecule spectroscopy at room temperature by laser-induced fluorescence with the use of fluorophores that are site-specifically attached to macromolecules is reviewed.
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Probing the interaction between two single molecules: fluorescence resonance energy transfer between a single donor and a single acceptor
Taekjip Ha,Th. Enderle,D. F. Ogletree,Daniel S. Chemla,Daniel S. Chemla,Paul R. Selvin,Paul R. Selvin,Shimon Weiss +7 more
TL;DR: In this article, the sensitivity of fluorescence resonance energy transfer (FRET) was extended to the single molecule level by measuring energy transfer between a single donor and a single acceptor fluorophore.
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The importance of repairing stalled replication forks
Michael M. Cox,Myron F. Goodman,Kenneth N. Kreuzer,David J. Sherratt,Steven J. Sandler,Kenneth J. Marians +5 more
TL;DR: The bacterial SOS response to unusual levels of DNA damage has been recognized and studied for several decades, but pathways for re-establishing inactivated replication forks under normal growth conditions have received far less attention.
Journal ArticleDOI
Analysis of Single-Molecule FRET Trajectories Using Hidden Markov Modeling
TL;DR: An analysis scheme is developed that casts single-molecule time-binned FRET trajectories as hidden Markov processes, allowing one to determine, based on probability alone, the most likely FRET-value distributions of states and their interconversion rates while simultaneously determining the mostlikely time sequence of underlying states for each trajectory.
Journal ArticleDOI
The structure of the E. coli recA protein monomer and polymer.
TL;DR: The crystal structure of the recA protein from Escherichia coli at 2.3-A resolution reveals a major domain that binds ADP and probably single-and double-stranded DNA as discussed by the authors.