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Ivan Rasnik
Researcher at Emory University
Publications - 6
Citations - 1250
Ivan Rasnik is an academic researcher from Emory University. The author has contributed to research in topics: Brownian ratchet & Folding (chemistry). The author has an hindex of 6, co-authored 6 publications receiving 1172 citations. Previous affiliations of Ivan Rasnik include University of Illinois at Urbana–Champaign.
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Nonblinking and long-lasting single-molecule fluorescence imaging
TL;DR: Trolox in combination with the enzymatic oxygen-scavenging system eliminates Cy5 blinking, dramatically reduces photobleaching and improves the signal linearity at high excitation rates, significantly extending the applicability of single-molecule fluorescence techniques.
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Real-time observation of RecA filament dynamics with single monomer resolution.
TL;DR: Using single-molecule fluorescence assays and hidden Markov modeling, the most direct evidence is shown that a RecA filament grows and shrinks primarily one monomer at a time and only at the extremities, supporting the proposal for a passive role of RecA-loading machineries in SSB removal.
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Dissecting metal ion–dependent folding and catalysis of a single DNAzyme
TL;DR: Analysis of metal-dependent folding and cleavage activity of the 8-17 DNAzyme using single-molecule fluorescence resonance energy transfer suggests that DNAzymes can use all modes of activation that metalloproteins use.
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Conformational trapping of Mismatch Recognition Complex MSH2/MSH3 on repair-resistant DNA loops
Walter H. Lang,Julie E. Coats,Jerzy Majka,Greg L. Hura,Yuyen Lin,Ivan Rasnik,Cynthia T. McMurray +6 more
TL;DR: It is reported that the mismatch recognition complex, MSH2/MSH3, discriminates between a Repair-competent and a repair-resistant loop by sensing the conformational dynamics of their junctions.
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Branch migration enzyme as a Brownian ratchet
Ivan Rasnik,Ivan Rasnik,Yong-Joo Jeong,Yong-Joo Jeong,Sean A. McKinney,Sean A. McKinney,Vaishnavi Rajagopal,Smita S. Patel,Taekjip Ha,Taekjip Ha +9 more
TL;DR: The results suggest that instead of actively unwinding base pairs as previously thought, the helicase exploits the spontaneous random walk of the junction and acts as a Brownian ratchet, which walks along duplex DNA while facilitating and biasing branch migration in a specific direction.