S
Sean A. McKinney
Researcher at University of Illinois at Urbana–Champaign
Publications - 17
Citations - 4723
Sean A. McKinney is an academic researcher from University of Illinois at Urbana–Champaign. The author has contributed to research in topics: Holliday junction & Branch migration. The author has an hindex of 13, co-authored 14 publications receiving 4459 citations. Previous affiliations of Sean A. McKinney include Howard Hughes Medical Institute.
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Journal ArticleDOI
Myosin V Walks Hand-Over-Hand: Single Fluorophore Imaging with 1.5-nm Localization
Ahmet Yildiz,Joseph N. Forkey,Sean A. McKinney,Taekjip Ha,Taekjip Ha,Yale E. Goldman,Paul R. Selvin,Paul R. Selvin +7 more
TL;DR: The results strongly support a hand-over-hand model of motility, not an inchworm model, which moves processively on actin.
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Analysis of Single-Molecule FRET Trajectories Using Hidden Markov Modeling
TL;DR: An analysis scheme is developed that casts single-molecule time-binned FRET trajectories as hidden Markov processes, allowing one to determine, based on probability alone, the most likely FRET-value distributions of states and their interconversion rates while simultaneously determining the mostlikely time sequence of underlying states for each trajectory.
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Nonblinking and long-lasting single-molecule fluorescence imaging
TL;DR: Trolox in combination with the enzymatic oxygen-scavenging system eliminates Cy5 blinking, dramatically reduces photobleaching and improves the signal linearity at high excitation rates, significantly extending the applicability of single-molecule fluorescence techniques.
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Structural dynamics of individual Holliday junctions.
TL;DR: Relative rates indicate that multiple conformer transitions occur at each intermediate step of branch migration, allowing the junction to reach conformational equilibrium, providing a mechanism whereby the sequence-dependent conformational bias could determine the extent of genetic exchange upon junction resolution.
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Real-time observation of RecA filament dynamics with single monomer resolution.
TL;DR: Using single-molecule fluorescence assays and hidden Markov modeling, the most direct evidence is shown that a RecA filament grows and shrinks primarily one monomer at a time and only at the extremities, supporting the proposal for a passive role of RecA-loading machineries in SSB removal.