Sean R. Cutler

TL;DR: A new model for ABA action has been proposed and validated, in which the soluble PYR/PYL/RCAR receptors function at the apex of a negative regulatory pathway to directly regulate PP2C phosphatases, which in turn directly regulate SnRK2 kinases.

TL;DR: PYR/PYLs are ABA receptors functioning at the apex of a negative regulatory pathway that controls ABA signaling by inhibiting PP2Cs, illustrating the power of the chemical genetic approach for sidestepping genetic redundancy.

TL;DR: This work shows that by combining the recently identified ABA receptor PYR1 with the type 2C protein phosphatase (PP2C) ABI1, the serine/threonine protein kinase SnRK2, and the transcription factor ABF2/AREB1, it can reconstitute ABA-triggered phosphorylation of the transcription factors in vitro and define a minimal set of core components of a complete major ABA signalling pathway.

TL;DR: The results indicate that screening GFP-fusion protein libraries is a useful approach for identifying and visualizing components of subcellular structures and their associated dynamics in higher plant cells.

TL;DR: It is concluded that ethylene appears to be a negative regulator of ABA action during germination, while the ethylene response pathway positively regulates some aspects of A BA action that involve root growth in the absence of ethylene.